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| Product Name | Mouse CD3+ T Cell Isolation Kit |
| Catalog No. | ATR-SPS-0043 |
| Description | A column-free magnetic negative-selection kit for isolating CD3+ pan-T-cell populations from mouse lymphoid tissues. Biotinylated antibodies and streptavidin magnetic beads remove non-target cells while the desired T cells remain unlabeled. |
| Intended Use | Isolation of mouse CD3+ T cells for culture, immunophenotyping and functional studies. |
| Principle / Technology | Negative selection using a biotinylated antibody cocktail and streptavidin magnetic beads. |
| Detection Method | Magnetic cell separation followed by flow-cytometric purity assessment. |
| Sample Type | Mouse spleen, lymph node and mixed lymphoid-tissue single-cell suspensions. |
| Performance Range / Specifications | Typical separation time: approximately 16 minutes; reported purity: 95-99%; recommended processing range: 1 × 10^7 to 2 × 10^8 cells at approximately 1 × 10^8 cells/mL; package capacity: up to 1 × 10^9 cells. |
| Reaction Conditions / Protocol | Incubate the suspension with the negative-selection antibody cocktail, add streptavidin magnetic beads and recover the unlabeled CD3+ T-cell fraction after magnetic separation. |
| Components / Formulation | Biotinylated cell-isolation antibody cocktail and streptavidin magnetic beads. |
| Storage Conditions | Store at 2-8°C. Do not freeze. |
| Package Specifications | For up to 1 × 10^9 cells. |
| Product Form | Column-free magnetic cell-isolation kit. |
| Key Features | Approximately sixteen-minute workflow; 95-99% reported purity; bead-free target cells; no separation column; high viability and yield; no abnormal activation. |
| Shipping Conditions | Ship with ice packs. |
| Compatibility | Compatible magnetic separators, flow cytometry, cell culture and T-cell functional assays. |
| Application Notes / Precautions | Use freshly prepared, aggregate-free single-cell suspensions and avoid excessive processing time or mechanical stress. |
For research use only, not for clinical use.
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