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| Product Name | Mouse CD3ε Nanobeads Kit |
| Catalog No. | RESK-0456 |
| Description | The Mouse CD3ε Nanobeads Kit is used for the isolation of mouse T cells based on CD3ε antigen expression. A biotinylated anti-mouse CD3ε antibody specifically binds CD3ε+ cells, followed by incubation and labeling with Anti-Biotin Nanobeads. The magnetically labeled cell suspension is then loaded onto an MS column or LS column, or another suitable separation column, placed in the separation magnet of a mainstream magnetic separation system. After magnetic separation, the cells retained in the column are the positively selected CD3ε-expressing cells, which can be used for downstream research; the flow-through can also be collected as the negatively selected cells without CD3ε expression. |
| Intended Use | Isolation of mouse CD3ε+ T cells based on CD3ε antigen expression. |
| Principle / Technology | A biotinylated anti-mouse CD3ε antibody specifically binds CD3ε+ cells; Anti-Biotin Nanobeads then magnetically label the antibody-bound cells. During column-based magnetic separation, labeled cells are retained in the column (positive selection) while unlabeled cells flow through (negative selection fraction). |
| Sample Type | Mouse cells (e.g., C57BL/6 mouse spleen cells) |
| Performance Range / Specifications | Sorting capacity: 10 T: up to 1×10^8 cells 50 T: up to 5×10^8 cells 100 T: up to 1×10^9 cells Bead particle size: 50-100 nm, equivalent to mainstream commercial products, with low toxicity |
| Specificity | The biotinylated hamster anti-mouse CD3ε antibody specifically binds mouse CD3ε antigen. |
| Reaction Conditions / Protocol | 1) Incubate mouse cells with the biotinylated hamster anti-mouse CD3ε antibody to specifically bind CD3ε+ cells. 2) Add Anti-Biotin Nanobeads and incubate to magnetically label the cells. 3) Load the magnetically labeled cell suspension onto an MS column or LS column, or another suitable separation column, placed in the separation magnet of a magnetic separation system. 4) After magnetic separation, the cells retained in the column are the positively selected CD3ε-expressing cells; the flow-through contains the negatively selected CD3ε-negative cells. |
| Components / Formulation | Biotin Hamster Anti-Mouse CD3ε Antibody: 15 μL (10 T) / 75 μL (50 T) / 150 μL (100 T) Anti-Biotin Nanobeads: 0.1 mL (10 T) / 0.5 mL (50 T) / 1 mL (100 T) Note: The Anti-Biotin Nanobeads included in this kit are also available separately. |
| Storage Conditions | Store at 2-8 °C protected from light. |
| Shelf Life | 6 months |
| Package Specifications | 10 T 50 T 100 T |
| Product Form | Kit containing biotinylated antibody and magnetic nanobead suspension |
| Quality Control | When CD3ε+ T cells are sorted from C57BL/6 mouse spleen cells with this kit, the purity, recovery, and viability of the sorted cells are comparable to those obtained with mainstream commercial magnetic bead kits. |
| Purity | High purity of sorted cells (comparable to mainstream commercial magnetic bead kits) |
| Source / Origin | Biotinylated hamster anti-mouse CD3ε antibody |
| Expiration Date / Stability | Valid for 6 months when stored at 2-8 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with MS columns, LS columns, and other suitable separation columns used with the separation magnets of mainstream magnetic separation systems. For optimal separation results, this kit can be used together with MS columns, a single-channel separation magnet, or a universal sorting stand from Alta DiagnoTech. |
| Application Notes / Precautions | High safety: bead particle size (50-100 nm) is equivalent to mainstream commercial products, with low toxicity. Excellent performance: high purity, high activity, and high recovery. Convenient operation: biodegradable matrix with no need for additional bead removal. High quality: high stability and high compatibility. For optimal separation results, this product can be used together with MS columns, a single-channel separation magnet, or a universal sorting stand from Alta DiagnoTech. |
| Recognition Site | Mouse CD3ε antigen |
For research use only, not for clinical use.
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