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| Product Name | Mouse CD19 Nanobeads |
| Catalog No. | RESK-0461 |
| Description | Mouse CD19 Nanobeads are designed for the isolation of mouse cells based on CD19 antigen expression. CD19 is a glycoprotein specifically expressed on the surface of B cells and is a member of the immunoglobulin superfamily. It is expressed from the B-cell precursor stage until it is downregulated upon differentiation into plasma cells. CD19 participates in regulating BCR signal transduction and influences B-cell proliferation, differentiation, and antibody production. CD19 is also highly expressed in most B-cell malignancies, such as B-cell lymphoma and acute lymphoblastic leukemia, but is not expressed in hematopoietic stem cells or non-B-cell tissues, making it a safe target for tumor immunotherapy. Mouse CD19 Nanobeads are prepared by conjugating a CD19 monoclonal antibody to nanoscale magnetic beads. Depending on the CD19 expression on the cell surface, they can be used to enrich or deplete CD19+ cells. The mouse CD19 sorting beads bind directly to target cells, after which the cell-bead mixture is loaded onto an LS column or another suitable separation column placed in the separation magnet of a magnetic separator. After magnetic separation, the cells retained in the column are the positively selected CD19-expressing cells, ready for downstream experiments. The flow-through can also be collected as the negatively selected fraction of cells not expressing CD19. |
| Intended Use | For magnetic enrichment or depletion of CD19-expressing mouse cells (e.g., B cells) for downstream research applications. |
| Principle / Technology | Positive selection by column-based magnetic separation: cells are directly labeled with anti-CD19 antibody-conjugated nanobeads and loaded onto a separation column placed in a magnetic separator. CD19+ cells are retained in the magnetized column (positive fraction), while unlabeled cells pass through and can be collected as the negative fraction. |
| Sample Type | Mouse cells, such as mouse spleen cells. |
| Performance Range / Specifications | High safety: bead particle size (50-100 nm) comparable to mainstream imported products, with low toxicity. Excellent performance: high purity, high viability, and high recovery. Convenient operation: biodegradable matrix, no additional bead removal required. High quality: high stability and high compatibility. Separation capacity: 10 T (up to 1×10^8 cells), 50 T (up to 5×10^8 cells), 100 T (up to 1×10^9 cells). |
| Specificity | Specific for the mouse CD19 antigen, a glycoprotein expressed on the B-cell surface. |
| Reaction Conditions / Protocol | Incubate the cells directly with the nanobeads, then load the cell-bead suspension onto an LS column or another suitable separation column placed in the separation magnet of a magnetic separator. After magnetic separation, the CD19+ cells retained in the column are collected as the positive fraction; the flow-through is collected as the CD19-negative fraction. |
| Components / Formulation | Matrix: dextran nanobeads Ligand: anti-mouse CD19 antibody Particle size: 50-100 nm |
| Storage Conditions | Store at 2-8 °C protected from light. |
| Shelf Life | 6 months when stored at 2-8 °C protected from light. |
| Package Specifications | 10 T / 50 T / 100 T (for up to 1×10^8 / 5×10^8 / 1×10^9 cells, respectively). |
| Product Form | Liquid suspension of dextran-based magnetic nanobeads. |
| Concentration | Separation capacity: up to 1×10^9 cells/mL of beads. |
| pH Range / Optimal pH | Storage buffer pH 7.2-7.4. |
| Expiration Date / Stability | Stable for 6 months when stored at 2-8 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with LS columns or other suitable separation columns used with mainstream magnetic separation systems; high compatibility with downstream experimental workflows. |
| Recommended Buffer System | Storage buffer: 1X PBS (pH 7.2-7.4) containing 2 mM EDTA and 0.5% BSA, Ca2+-free. |
| Application Notes / Precautions | Protect from light during storage. The nanobeads use a biodegradable matrix, so no additional bead removal step is required after separation. The purity, recovery, and viability of cells isolated from mouse spleen cells are comparable to those obtained with mainstream imported products. |
For research use only, not for clinical use.
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