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| Product Name | Mouse B Cell Isolation Kit |
| Catalog No. | ATR-SPS-0040 |
| Description | A column-free magnetic negative-selection kit for enriching B cells from mouse lymphoid tissues. Biotinylated antibodies label unwanted cells, and streptavidin magnetic beads remove the labeled fraction without directly binding the target B cells. |
| Intended Use | Isolation of B cells from mouse spleen and lymph-node single-cell suspensions. |
| Principle / Technology | Negative selection using a biotinylated antibody cocktail and streptavidin-coated magnetic beads. |
| Detection Method | Magnetic cell separation followed by flow-cytometric purity assessment. |
| Sample Type | Mouse spleen, lymph node and mixed spleen/lymph-node single-cell suspensions. |
| Performance Range / Specifications | Typical separation time: approximately 16 minutes; reported purity: up to 99%; recommended processing range: 1 × 10^7 to 2 × 10^8 cells at approximately 1 × 10^8 cells/mL; package capacity: up to 1 × 10^9 cells. |
| Reaction Conditions / Protocol | Label non-target cells with the antibody cocktail, incubate with streptavidin magnetic beads and collect the unlabeled B-cell fraction after magnetic separation. |
| Components / Formulation | Biotinylated cell-isolation antibody cocktail and streptavidin magnetic beads. |
| Storage Conditions | Store at 2-8°C. Do not freeze. |
| Package Specifications | For up to 1 × 10^9 cells. |
| Product Form | Column-free magnetic cell-isolation kit. |
| Key Features | Purity up to 99%; approximately sixteen-minute workflow; no separation column; bead-free target cells; maintained viability and yield; broad lymphoid-sample compatibility. |
| Shipping Conditions | Ship with ice packs. |
| Compatibility | Compatible magnetic separators, flow cytometry, cell culture and downstream functional assays. |
| Application Notes / Precautions | Filter out cell aggregates before separation, mix magnetic beads thoroughly during incubation and keep the workflow cold to preserve cell viability. |
For research use only, not for clinical use.
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