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| Product Name | Monoamine Oxidase (MAO) Activity Fluorometric Assay Kit |
| Catalog No. | ETR-HMM-0079 |
| Description | A fluorometric assay designed for the detection of monoamine oxidase activity in tissue and cell samples. The assay employs a synthetic amine substrate that is oxidatively deaminated by MAO, producing a fluorescent aldehyde product detected directly without secondary enzymatic coupling steps. Separate MAO-A and MAO-B isoform detection is possible using isoform-selective substrates or inhibitors included in the kit. |
| Intended Use | Quantification of total and isoform-specific monoamine oxidase enzymatic activity in biological specimens. |
| Principle / Technology | Direct fluorometric detection of aldehyde produced by MAO-catalyzed oxidative deamination |
| Detection Method | Fluorescence (excitation 365 nm, emission 450 nm) |
| Sample Type | Tissue homogenates (brain, liver, kidney), cell lysates, mitochondrial fractions |
| Performance Range / Specifications | Detection range: 0.1–10 mU per well; reaction time: 30–60 min at 37 °C |
| Sensitivity / LOD | Detection limit: 0.05 mU per well |
| Specificity | MAO-A selective substrate: serotonin analog; MAO-B selective substrate: benzylamine analog; inhibitors: clorgyline (MAO-A) and pargyline (MAO-B) for isoform discrimination |
| Reaction Conditions / Protocol | Prepare sample in assay buffer; add selective substrate or total MAO substrate; incubate 30–60 min at 37 °C; read fluorescence |
| Components / Formulation | Total MAO substrate (100×), MAO-A selective substrate (100×), MAO-B selective substrate (100×), clorgyline inhibitor, pargyline inhibitor, aldehyde standard, assay buffer |
| Storage Conditions | –20 °C for substrates and standards; 2–8 °C for buffer |
| Shelf Life | 6 months |
| Package Specifications | 100 tests |
| Product Form | Liquid reagents and lyophilized standards |
| Quality Control | Validated with rat liver mitochondrial extract; isoform discrimination confirmed with known inhibitor responses |
| Key Features | Direct fluorometric detection eliminates coupled-enzyme variability; isoform-selective substrates enable MAO-A/MAO-B differentiation |
| Purity | Reagent grade; substrate purity ≥98% by HPLC where applicable |
| Concentration | As specified per kit; calibrator and substrate concentrations traceable to reference |
| Activity / Unit Definition | One unit defined as the amount of enzyme that catalyzes conversion of 1 µmol substrate per minute under specified conditions |
| Molecular Weight | Varies by target enzyme as documented |
| Source / Origin | Recombinant or purified from natural sources; synthetic chromogenic/fluorogenic substrates |
| pH Range / Optimal pH | pH 6.0–9.0 depending on specific enzyme assay |
| Shipping Conditions | Cold packs for enzyme components; ambient for buffers |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 quality system |
| Compatibility | Compatible with standard microplate readers (absorbance, fluorescence, luminescence modes) |
| Recommended Buffer System | Tris or phosphate buffer optimized per enzyme assay |
| Application Notes / Precautions | Include positive control and inhibitor control in each assay; read within linear range; protect light-sensitive substrates |
| Batch-to-Batch Consistency | Enzyme activity and standard curve linearity within ±15% of reference lot |
For research use only, not for clinical use.
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