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| Product Name | Elastase Activity Fluorometric Assay Kit |
| Catalog No. | ETR-HMM-0081 |
| Description | A fluorometric assay kit designed to measure neutrophil elastase activity in biological samples and inhibitor screening applications. The assay uses a peptide substrate conjugated to a fluorophore whose fluorescence is quenched in the intact state by a nearby quencher molecule. Upon elastase-mediated cleavage of the peptide linker, the fluorophore is released from quenching and emits a quantitative fluorescent signal. |
| Intended Use | Measurement of neutrophil elastase activity in biological fluids and cell lysates, with optional inhibitor profiling capability. |
| Principle / Technology | FRET-based peptide substrate cleavage with fluorescence dequenching |
| Detection Method | Fluorescence (excitation 490 nm, emission 520 nm) |
| Sample Type | Bronchoalveolar lavage fluid, sputum, neutrophil lysates, cell culture supernatant |
| Performance Range / Specifications | Linear range: 0.01–2.0 mU per well; reaction kinetics readable in continuous mode |
| Sensitivity / LOD | Detection limit: 0.005 mU per well |
| Specificity | Substrate cleaved by neutrophil elastase, proteinase 3; minimal cleavage by cathepsin G; specificity confirmed with selective elastase inhibitor (elastatinal) |
| Reaction Conditions / Protocol | Add sample to well; add substrate working solution; read fluorescence kinetically at 37 °C for 30–60 min; fluorescence increase rate proportional to activity |
| Components / Formulation | FRET peptide substrate (lyophilized), elastase positive control, elastatinal inhibitor, reaction buffer, fluorophore reference standard |
| Storage Conditions | –20 °C for substrate, enzyme and inhibitor; 2–8 °C for buffer |
| Shelf Life | 6 months at –20 °C |
| Package Specifications | 100 tests |
| Product Form | Lyophilized substrate, liquid reagents |
| Quality Control | Functional validation with purified human neutrophil elastase; inhibitor control reduces signal ≥90% |
| Key Features | FRET format provides low background and high signal-to-noise ratio, suitable for both endpoint and kinetic reading |
| Purity | Reagent grade; substrate purity ≥98% by HPLC where applicable |
| Concentration | As specified per kit; calibrator and substrate concentrations traceable to reference |
| Activity / Unit Definition | One unit defined as the amount of enzyme that catalyzes conversion of 1 µmol substrate per minute under specified conditions |
| Molecular Weight | Varies by target enzyme as documented |
| Source / Origin | Recombinant or purified from natural sources; synthetic chromogenic/fluorogenic substrates |
| pH Range / Optimal pH | pH 6.0–9.0 depending on specific enzyme assay |
| Shipping Conditions | Cold packs for enzyme components; ambient for buffers |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 quality system |
| Compatibility | Compatible with standard microplate readers (absorbance, fluorescence, luminescence modes) |
| Recommended Buffer System | Tris or phosphate buffer optimized per enzyme assay |
| Application Notes / Precautions | Include positive control and inhibitor control in each assay; read within linear range; protect light-sensitive substrates |
| Batch-to-Batch Consistency | Enzyme activity and standard curve linearity within ±15% of reference lot |
For research use only, not for clinical use.
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