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Modified Ringer's Solution (10× MMR), pH 7.7

Cat.No: RERE-0410 Datasheet

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Product Name Modified Ringer's Solution (10× MMR), pH 7.7
Catalog No. RERE-0410
Description Marc's Modified Ringer solution is the amphibian workbench standard, and this tenfold concentrate carries sodium chloride, potassium chloride, HEPES and EDTA so that a diluted stock is always one step away. In interphase extraction experiments, where oocytes are cleaned before their contents are manipulated, the EDTA component restrains nucleases while HEPES holds pH at 7.7. Dilute to anywhere between 0.25× and 1× as the protocol demands.
Intended Use Cleaning of oocytes for interphase extraction and general amphibian cell handling from a concentrated stock.
Principle / Technology HEPES-buffered physiological salinity with EDTA preserves cells and restrains nuclease activity.
Specificity 10× concentrate; composed of sodium chloride, potassium chloride, HEPES and EDTA; pH 7.7.
Reaction Conditions / Protocol Dilute to 0.25-1× with water before use, according to the experimental requirement.
Storage Conditions 2-8°C; 6 months.
Package Specifications 100 mL, 500 mL
Key Features Tenfold concentrate with flexible working range; EDTA protected; two bottle sizes.

For research use only, not for clinical use.

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