Model Species rRNA Depletion Kit

Name: Model Species rRNA Depletion Kit
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Cat.No: NG-QCY-0015 Datasheet

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Product Details Related Products

Product Name	Model Species rRNA Depletion Kit
Catalog No.	NG-QCY-0015
Description	An RNase H-based depletion kit for removing ribosomal RNA from total RNA of commonly studied model organisms.
Intended Use	mRNA and noncoding RNA analysis from zebrafish, nematodes, fruit flies, ciliates and other supported model species.
Principle / Technology	Species-targeted probes hybridize to rRNA, followed by RNase H digestion and DNase I treatment to remove rRNA and residual probes while retaining mRNA and noncoding RNA.
Detection Method	Downstream RNA-seq library preparation and sequencing.
Sample Type	Intact or partially degraded total RNA from supported model species.
Performance Range / Specifications	Input range 0.01–1 µg total RNA; 6, 12 or 24 reactions; approximately 1-hour workflow.
Sensitivity / LOD	Reported residual rRNA below 1%.
Specificity	Targets 5S, 5.8S, 18S, 28S, 12S, 16S and 45S rRNA while preserving mRNA and other noncoding RNA.
Reaction Conditions / Protocol	Hybridize total RNA with depletion probes, digest RNA–DNA hybrids with RNase H, remove probe DNA with DNase I and purify the depleted RNA.
Components / Formulation	Species-targeted rRNA depletion probes, RNase H digestion reagents and DNase I treatment reagents.
Storage Conditions	Store at −30 to −15°C.
Package Specifications	6 reactions; 12 reactions; 24 reactions.
Product Form	Multi-component rRNA depletion kit.
Quality Control	Residual rRNA, species compatibility, mapping rate and downstream RNA-seq performance are evaluated.
Key Features	Broad model-species coverage; below 1% residual rRNA; approximately 1-hour workflow; compatible with degraded RNA.
Shipping Conditions	Ship at or below 0°C.
Compatibility	Suitable for downstream coding and noncoding RNA sequencing workflows.

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For research use only, not for clinical use.