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| Product Name | Methemoglobin (MetHb) Content Assay Reagent (Visible Spectrophotometer) |
| Catalog No. | PTR-HMM-0043 |
| Description | Methemoglobin (MetHb) is an oxidized form of hemoglobin, formed when the iron ions in the heme group of deoxygenated or oxygenated hemoglobin are completely or partially oxidized from ferrous ions to ferric ions. After oxidation, methemoglobin loses its oxygen-carrying capacity. The level of methemoglobin can assess the oxygen-carrying capacity of blood and red blood cell substitutes, making it of great significance in the clinical diagnosis of methemoglobinemia and the development of red blood cell substitutes. |
| Application | Hemoglobin can bind with oxygen to form oxygenated hemoglobin, and upon deoxygenation, it becomes deoxygenated hemoglobin. By measuring the absorbance values of oxygenated hemoglobin, deoxygenated hemoglobin, and methemoglobin at 560 nm, 576 nm, and 630 nm, respectively, and using the extinction coefficients of each component at specific wavelengths, the mass concentration percentage of methemoglobin can be calculated. After determining the total hemoglobin content in the sample, the methemoglobin content can be calculated. |
| Applicable Instruments | Visible Spectrophotometer |
| Number of Testable Samples | 48 Samples |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Detection Time | 2 h (48 Samples) |
| Detection Method | Hemoglobin Determination |
| Spectral Parameters | 560 nm; 576 nm; 630 nm |
| Signal Response | Incremental |
| Notes | If the total A measurement is greater than 1.0, it is recommended to dilute the sample with distilled water before performing the measurement; if the total A measurement is less than 0.02, it is recommended to increase the sample volume and then perform the measurement, making corresponding adjustments during calculation. |
For research use only, not for clinical use.
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