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| Product Name | Free Hemoglobin (FHb) Content Assay Reagent (Microplate Reader) |
| Catalog No. | PTR-HMM-0042 |
| Description | Free hemoglobin (FHb) refers to free hemoglobin molecules in plasma that are not bound to red blood cell membranes. When hemolysis occurs, red blood cells rupture, releasing hemoglobin and increasing the concentration of free hemoglobin in plasma. Changes in its concentration play a significant role in cellular immunity, trauma treatment, and the diagnosis of hemolytic diseases. |
| Application | The ferrous heme in hemoglobin has similar catalytic activity to peroxidase, which can catalyze the oxidation of 4-aminoantipyrine and aniline analogues by hydrogen peroxide to form purple compounds. The products have a characteristic absorption peak at 546 nm, and the content of free hemoglobin can be quantitatively detected by measuring the change in absorbance. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 96 Samples |
| Matching | 96-well plate |
| Detection Time | 3 h (96 Samples) |
| Detection Method | 4-Aminoantipyrine |
| Spectral Parameters | 546 nm |
| Signal Response | Incremental |
| Notes | Avoid hemolysis during sampling and plasma separation; if the A measurement is greater than 1.0, it is recommended to dilute the sample with distilled water before testing; If the A measurement is less than 0.02, it is recommended to increase the sample volume during sample preparation, re-extract the sample, and then perform the measurement, making corresponding adjustments during calculation. |
For research use only, not for clinical use.
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