Free Hemoglobin (FHb) Content Assay Reagent (Microplate Reader)
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Free Hemoglobin (FHb) Content Assay Reagent (Microplate Reader)

Cat.No: PTR-HMM-0042 Datasheet

Specification Quantities

100T/96S:
- +
Product Details Related Products
Product Name Free Hemoglobin (FHb) Content Assay Reagent (Microplate Reader)
Catalog No. PTR-HMM-0042
Description Free hemoglobin (FHb) refers to free hemoglobin molecules in plasma that are not bound to red blood cell membranes. When hemolysis occurs, red blood cells rupture, releasing hemoglobin and increasing the concentration of free hemoglobin in plasma. Changes in its concentration play a significant role in cellular immunity, trauma treatment, and the diagnosis of hemolytic diseases.
Application The ferrous heme in hemoglobin has similar catalytic activity to peroxidase, which can catalyze the oxidation of 4-aminoantipyrine and aniline analogues by hydrogen peroxide to form purple compounds. The products have a characteristic absorption peak at 546 nm, and the content of free hemoglobin can be quantitatively detected by measuring the change in absorbance.
Applicable Instruments Microplate Reader
Number of Testable Samples 96 Samples
Matching 96-well plate
Detection Time 3 h (96 Samples)
Detection Method 4-Aminoantipyrine
Spectral Parameters 546 nm
Signal Response Incremental
Notes Avoid hemolysis during sampling and plasma separation; if the A measurement is greater than 1.0, it is recommended to dilute the sample with distilled water before testing; If the A measurement is less than 0.02, it is recommended to increase the sample volume during sample preparation, re-extract the sample, and then perform the measurement, making corresponding adjustments during calculation.

For research use only, not for clinical use.

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