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| Product Name | Precision Plus Protein Dual Color Standards (Kaleidoscope) |
| Catalog No. | PTR-HMM-0078 |
| Description | A mixture of ten recombinant proteins with molecular weights from 10 to 250 kDa, each pre-stained with distinct colors for direct visualization during SDS-PAGE and Western blotting. The standards include three reference bands of higher intensity. |
| Intended Use | Molecular weight estimation of proteins separated by SDS-PAGE and monitoring of electrophoretic protein transfer to membranes in Western blotting. |
| Principle / Technology | Proteins of precisely known molecular weight are covalently coupled to chromophores producing distinct colored bands; migration distance on SDS-PAGE is logarithmically related to molecular weight. |
| Detection Method | Visual tracking during electrophoresis and transfer; imaging with standard gel documentation systems under white light or UV transillumination |
| Sample Type | Standards are loaded directly onto SDS-PAGE gels alongside experimental samples |
| Performance Range / Specifications | Molecular weight range: 10, 15, 20, 25, 37, 50, 75, 100, 150, and 250 kDa bands; three reference bands (25, 50, and 75 kDa) exhibit increased intensity for orientation |
| Sensitivity / LOD | Approximately 0.5–1 μg per band is visible by direct observation |
| Specificity | Each band corresponds to a single, purified recombinant protein of defined molecular weight |
| Reaction Conditions / Protocol | Thaw vial at room temperature, vortex briefly, load 5–10 μL per lane on SDS-PAGE gel, run at constant voltage until dye front reaches the bottom of the gel; for Western blotting, standards transfer to membrane and remain visible |
| Components / Formulation | Pre-stained protein standard mixture in SDS-PAGE loading buffer with glycerol, provided in ready-to-load format |
| Storage Conditions | -20°C for long-term storage; 2–8°C for working aliquots (stable for 3 months); avoid repeated freeze-thaw cycles |
| Shelf Life | 12 months from date of manufacture at -20°C |
| Package Specifications | 500 μL (sufficient for 50–100 gel lanes) |
| Product Form | Ready-to-load liquid in SDS-PAGE sample buffer |
| Quality Control | Apparent molecular weight of each band verified by SDS-PAGE against unstained molecular weight markers; lot-specific molecular weight values provided for each band |
| Key Features | Multicolor format enables instant band identification and orientation during electrophoresis; pre-stained bands remain visible on membranes after transfer, confirming transfer efficiency; the 250 kDa band provides a high-end molecular weight reference |
For research use only, not for clinical use.
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