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| Product Name | ELISA Development Kit with Coating Buffer Set |
| Catalog No. | PTR-HMM-0067 |
| Description | A comprehensive ELISA development kit containing matched antibody pairs, coating buffer, blocking solution, and detection reagents for building custom sandwich ELISA assays. This flexible system allows laboratories to develop quantitative immunoassays tailored to their specific target protein. |
| Intended Use | Development of custom sandwich ELISA protocols for quantitative protein detection in biological samples, providing flexibility in assay design and optimization. |
| Principle / Technology | Sandwich ELISA format with capture antibody immobilized on the microplate surface, target protein binding, and detection antibody with enzymatic signal generation. |
| Detection Method | Colorimetric detection at 450 nm after TMB substrate development |
| Sample Type | Cell culture supernatant, serum, plasma, purified recombinant proteins, tissue lysates |
| Performance Range / Specifications | Assay range is dependent on the selected antibody pair and target protein; typical dynamic ranges span 2–3 orders of magnitude |
| Sensitivity / LOD | Sensitivity is determined by the affinity of the selected antibody pair and can reach the low picogram per milliliter range |
| Specificity | Specificity is determined by the selected capture and detection antibody pair; recommendations for matched pairs with validated specificity are provided |
| Reaction Conditions / Protocol | Coat plate with capture antibody overnight at 4°C, block with blocking buffer for 1 hour, add sample and incubate 2 hours at room temperature, add detection antibody for 1 hour, add streptavidin-HRP for 30 minutes, develop with TMB and stop |
| Components / Formulation | Capture antibody (purified), biotinylated detection antibody, protein standard (recombinant), coating buffer concentrate, blocking buffer, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer, detailed protocol guide |
| Storage Conditions | Capture and detection antibodies at -20°C; buffer reagents at 2–8°C; reconstituted standard at -20°C in single-use aliquots |
| Shelf Life | 18 months for buffer reagents; 24 months for lyophilized antibodies |
| Package Specifications | Sufficient reagents for 10 x 96-well plates when used at recommended concentrations |
| Product Form | Lyophilized antibodies with liquid buffer reagents |
| Quality Control | Matched antibody pairs are validated for sandwich ELISA performance; each lot tested for sensitivity, dynamic range, and matrix compatibility |
| Key Features | Flexible format allows assay customization and optimization; matched antibody pairs ensure specificity; comprehensive buffer set eliminates the need for separate reagent sourcing |
For research use only, not for clinical use.
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