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| Product Name | Micro BCA Protein Assay Kit, High-Sensitivity (0.5-20 µg/mL) |
| Catalog No. | PTR-HMM-0088 |
| Description | Micro BCA (bicinchoninic acid) protein assay kit for ultrasensitive total protein quantification down to 0.5 µg/mL. The Micro BCA chemistry uses Cu⁺ reduction of BCA with tartrate as a stabilizing chelator at elevated temperature (60 °C), dramatically increasing sensitivity compared to standard BCA and enabling detection of dilute protein samples from immunoprecipitation eluates, column chromatography fractions, and low-cell-number samples. |
| Intended Use | Ultrasensitive protein quantification (0.5-20 µg/mL); dilute protein sample quantification; immunoprecipitation eluate protein determination; chromatography fraction analysis; low-cell-number sample quantification; protein determination prior to mass spectrometry. |
| Principle / Technology | Proteins reduce Cu²⁺ to Cu⁺ in alkaline medium (Biuret reaction); Cu⁺ chelates with 2 molecules of bicinchoninic acid (BCA) forming purple complex with strong absorbance at 562 nm; Micro BCA: tartrate stabilizes Cu⁺ at 60 °C enabling 10× sensitivity improvement over standard BCA |
| Detection Method | Colorimetric; absorbance at 562 nm; incubation at 60 °C for 60 min; standard curve 0.5-20 µg/mL BSA |
| Sample Type | Dilute protein solutions; aqueous buffers; samples with low protein concentration (<20 µg/mL); compatible with 0.5-5% detergents after dilution |
| Sensitivity / LOD | Linear range 0.5-20 µg/mL BSA (standard protocol); detection limit 0.2 µg/mL; 10-fold more sensitive than standard BCA |
| Specificity | Protein quantification based on peptide bond-mediated Cu²⁺ reduction and Cys/Tyr/Trp residue contribution; chelating agents (EDTA >10 mM), reducing agents (DTT >1 mM), and ammonium sulfate (>25% saturation) interfere |
| Reaction Conditions / Protocol | Mix 0.5 mL sample with 0.5 mL Micro BCA working reagent (MA:MB:MC = 25:24:1); incubate at 60 °C for 60 min; cool to RT; read A562; compare to BSA standard curve |
| Components / Formulation | Micro BCA Reagent MA (sodium carbonate, sodium tartrate, sodium bicarbonate in 0.1 M NaOH), Micro BCA Reagent MB (BCA disodium salt in water), Micro BCA Reagent MC (copper(II) sulfate pentahydrate 4% solution), BSA standard set (2.0, 1.5, 1.0, 0.75, 0.5, 0.25, 0.125, 0 mg/mL), instruction manual |
| Storage Conditions | 15-25 °C; BSA standards at 4 °C; Reagent C (copper sulfate) stable at RT |
| Shelf Life | 12 months from manufacture date |
| Package Specifications | 500 assays, 1,000 assays; accommodates 1 mL cuvette or 200 µL microplate formats |
| Product Form | Liquid reagents; BSA standard set pre-diluted |
| Key Features | 10× more sensitive than standard BCA (0.5 vs. 5 µg/mL detection limit); compatible with common detergents (SDS, Triton X-100, NP-40) at working concentrations; simple mix-and-read protocol; pre-diluted BSA standards for convenience; 60 °C incubation minimizes time (1 hr vs. 2 hr for standard BCA at 37 °C) |
| Purity | BCA reagent purity >99%; BSA standards fatty acid-free, >98% pure; copper sulfate ACS grade |
| Concentration | As specified on product label or certificate of analysis |
| Activity / Unit Definition | Binding capacity or enzymatic activity as specified per product documentation |
| Molecular Weight | Varies by product as specified in documentation |
| Source / Origin | Recombinant expression in E. coli, mammalian, or insect cell systems; synthetic chemistry for small molecule reagents |
| pH Range / Optimal pH | pH 7.0–8.0 for most protein applications |
| Shipping Conditions | Ambient; BSA standards cold pack |
| Expiration Date / Stability | 12 months at RT for reagents; BSA standards 12 months at 4 °C; working reagent stable 24 hr at RT; discard Reagent MC if blue precipitate forms (Cu(OH)₂) |
| Regulatory / Compliance | For laboratory and research use only; RUO; Reagent MA contains NaOH — corrosive; ISO 9001 |
| Compatibility | Compatible with standard SDS-PAGE, Western blot, mass spectrometry, and chromatography systems |
| Recommended Buffer System | PBS, Tris, or HEPES buffers as specified in product protocol |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles for protein solutions; use fresh aliquots; protect light-sensitive reagents |
| Batch-to-Batch Consistency | Protein concentration and activity within ±15% of reference lot |
For research use only, not for clinical use.
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