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| Product Name | Magnetic Bead-Based Protein Enrichment Buffer System for Low-Abundance Target Capture |
| Catalog No. | ATR-SPS-0026 |
| Description | Optimized buffer system for magnetic bead immunoprecipitation including binding/wash buffer, acidic and SDS elution buffers, neutralization buffer, and bead regeneration buffer. Low-background formulation minimizes non-specific binding while maximizing antibody-antigen interactions. Compatible with diverse magnetic bead types for protein enrichment applications. |
| Intended Use | Magnetic bead IP and Co-IP; target protein enrichment from lysates; protein complex purification; biotinylated protein pull-down; phosphopeptide/ubiquitinated protein enrichment; pre-MS sample preparation. |
| Principle / Technology | Optimized 150 mM NaCl + 0.05% NP-40 balances specific binding against hydrophobic non-specific interactions; BSA-free avoids antibody binding site competition |
| Detection Method | Bind: antibody-beads + lysate in binding buffer (1-2 hr, 4 °C); Wash: 3-5× wash buffer; Elute: acid (pH 2.8) or SDS (2%) for 5-10 min; neutralize acid eluates |
| Sample Type | Cell lysates (1-5 mg protein per IP), tissue homogenates; pre-clear by 14,000×g centrifugation |
| Components / Formulation | Binding/Wash Buffer (Tris 50 mM, NaCl 150 mM, NP-40 0.05%, pH 7.4, 100 mL); Acidic Elution (Glycine-HCl 100 mM, pH 2.8, 25 mL); Neutralization (Tris 1 M, pH 8.5, 5 mL); SDS Elution (Tris 50 mM, SDS 2%, 25 mL); Regeneration (Glycine-HCl 100 mM, pH 2.5, 25 mL) |
| Storage Conditions | 4 °C binding/wash; RT elution/regeneration; protect SDS buffer from <15 °C |
| Shelf Life | 24 months from manufacture date |
| Package Specifications | Complete set for 50 IP reactions; individual buffers available |
| Product Form | Liquid buffers, ready-to-use |
| Key Features | Low-background BSA-free formulation; acid and SDS elution options; compatible with major magnetic bead types; bead regeneration extends lifetime 5-10×; EDTA-free version available |
| Purity | All components molecular biology grade; BSA: none detected; heavy metals <1 ppm; endotoxin <0.05 EU/mL |
| Concentration | As specified on product label |
| Activity / Unit Definition | Sample processing efficiency ≥90% recovery per validation protocol |
| Molecular Weight | Not applicable or as specified for enzyme components |
| Source / Origin | Synthetic and recombinant components as specified |
| pH Range / Optimal pH | Binding/Wash: pH 7.4±0.1; Acid Elution: pH 2.8±0.1; Neutralization: pH 8.5±0.1 |
| Shipping Conditions | Ambient; SDS may precipitate — warm to 30 °C |
| Expiration Date / Stability | 24 months; discard if turbid or microbial growth |
| Regulatory / Compliance | For laboratory and research use only; RUO; acid elution corrosive; ISO 9001 |
| Compatibility | Compatible with downstream PCR, mass spectrometry, and immunoassay workflows |
| Recommended Buffer System | Use as directed; components optimized for stated application |
| Application Notes / Precautions | Work quickly to prevent sample degradation; use appropriate protease/phosphatase inhibitors if needed |
| Batch-to-Batch Consistency | Protein precipitation efficiency and analyte recovery within ±15% of reference lot |
For research use only, not for clinical use.
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