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| Product Name | Magnetic Bead-Based DNA Purification Binding Buffer |
| Catalog No. | ATR-SPS-0018 |
| Description | A chaotropic salt-based binding buffer optimized for the selective binding of DNA to silica-coated magnetic beads in automated nucleic acid extraction platforms, ensuring high recovery and purity. |
| Intended Use | Binding buffer component in magnetic bead-based DNA extraction protocols for automated liquid handling platforms, enabling high-throughput purification of genomic DNA, PCR products, and plasmid DNA. |
| Principle / Technology | High concentration of guanidine hydrochloride or sodium iodide creates chaotropic conditions that promote DNA adsorption to silica surfaces through hydrogen bonding and hydrophobic interactions while proteins and other contaminants remain in solution. |
| Detection Method | DNA recovery by Qubit fluorometry, A260/A280 purity, agarose gel integrity |
| Sample Type | Whole blood, buffy coat, tissue lysates, bacterial cultures, PCR reactions |
| Performance Range / Specifications | DNA recovery >90% from 1 µg input; A260/A280 1.75–1.90; DNA fragment retention >100 bp |
| Sensitivity / LOD | N/A |
| Specificity | Selective for double-stranded DNA; minimal RNA binding |
| Reaction Conditions / Protocol | Mix sample lysate 1:1 with binding buffer; add magnetic beads; mix and capture beads on magnet; wash; elute in low-salt buffer or water. |
| Components / Formulation | Guanidine hydrochloride, isopropanol, Tris buffer pH 6.5, magnetic bead-compatible surfactant |
| Storage Conditions | 15–30°C; flammable due to isopropanol content |
| Shelf Life | 18 months |
| Package Specifications | 50 mL, 250 mL, 1 L |
| Product Form | Liquid, ready to use |
| Quality Control | DNA binding capacity, elution efficiency with standard DNA, DNase/RNase free, pH |
| Key Features | Optimized for magnetic bead platforms; high DNA recovery; ready to use; compatible with automated liquid handlers; validated for multiple sample types |
For research use only, not for clinical use.
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