| Product Name | Lowry Protein Content Assay Reagent |
| Catalog No. | PTR-HMM-0036 |
| Description | The Lowry protein content detection method involves the formation of a protein-copper complex under alkaline conditions, followed by the reduction of Folin reagent to form a deep blue compound. The product exhibits a characteristic absorption peak at 650 nm, and the protein concentration in the sample can be quantitatively detected by measuring the change in absorption value. |
| Application | The Lowry protein content detection method involves the formation of a protein-copper complex under alkaline conditions, followed by the reduction of Folin reagent to form a deep blue compound. The product has a characteristic absorption peak at 650 nm, and the protein concentration in the sample can be quantitatively determined by measuring the change in the absorbance value. |
| Applicable Instruments | Visible Spectrophotometer/Microplate Reader |
| Number of Testable Samples | 100 Samples |
| Matching | 1 mL glass cuvette (d=10 mm)/96-well plate |
| Detection Time | 3 h (100 Samples) |
| Detection Method | Lowry |
| Spectral Parameters | 650 nm |
| Signal Response | Incremental |
| Standard | BSA |
| Reference Standards | y=1.245x+0.0473 (R2=0.9997) |
| Standard Linear Range | 0.1-0.5 mg/mL |
| Detection Limit | 0.05 mg/mL |
| Notes | BSA Standard Solution is stable for 3 months at 4°C and for 1 year at -20°C. Other reagents are stable for 1 year at room temperature. Once opened, reagents should be stored in a tightly sealed container. The Lowry method has a quantification range of 50–500 µg/mL. The Folin reagent color reaction is caused by tyrosine, tryptophan, and cysteine. Samples containing phenols, citric acid, and thiol compounds may interfere with the reaction. Different proteins may exhibit slight variations in color intensity due to differences in tyrosine and tryptophan content. |
For research use only, not for clinical use.
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