| Product Name | Glycosylated Hemoglobin (GHb) Content Assay Reagent (Visible Spectrophotometer) |
| Catalog No. | PTR-HMM-0045 |
| Description | Glycated hemoglobin (GHb) is formed when hemoglobin in red blood cells reacts with sugars (primarily glucose) in serum. Glycated hemoglobin consists of HbA1a, HbA1b, and HbA1c, with HbA1c accounting for approximately 70% and having a relatively stable structure. The non-enzymatic reaction of glycated hemoglobin is characterized by being continuous, slow, and irreversible. Its concentration is determined by past rather than current blood glucose levels and is unaffected by factors such as fasting status, insulin injection, or the use of antidiabetic medications prior to testing. It is commonly used for monitoring diabetes control indicators. |
| Application | The sugar on glycated hemoglobin is heated under acidic conditions, undergoes dehydration and condensation to form 5-hydroxymethylfurfural (5-HMF), which reacts with TBA (Thiobarbituric Acid) to form a colored substance. The product has a characteristic absorption peak at 443 nm, and the content of glycated hemoglobin can be quantitatively detected by measuring the change in absorbance. |
| Applicable Instruments | Visible Spectrophotometer |
| Number of Testable Samples | 48 Samples |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Detection Time | 4 h (48 Samples) |
| Detection Method | TBA |
| Spectral Parameters | 443 nm |
| Signal Response | Incremental |
| Notes | When adding reagent three, mix thoroughly while adding. After adding part of reagent three, the reaction solution may solidify; stir thoroughly before centrifuging; If the total hemoglobin measurement absorbance value ΔA total is greater than 1.0, it is recommended to dilute the sample with distilled water before testing and adjust the calculation accordingly; if the total hemoglobin measurement absorbance value ΔA total is less than 0.02, it is recommended to increase the sample volume during sample preparation, re-extract, and then perform the measurement, adjusting the calculation accordingly; If the absorbance value ΔA measured for glycated hemoglobin is greater than 1.0, it is recommended to dilute the sample with distilled water before measurement and adjust the calculation accordingly. If the absorbance value ΔA measured for glycated hemoglobin is less than 0.02, it is recommended to increase the sample volume during sample preparation, re-extract the sample, and then perform the measurement, adjusting the calculation accordingly. |
For research use only, not for clinical use.
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