| Product Name | Bradford Protein Content Assay Reagent |
| Catalog No. | PTR-HMM-0035 |
| Description | The Bradford method for determining protein content is a type of dye binding method. Coomassie Brilliant Blue G-250 dye reaches equilibrium with protein within 2–5 minutes, and the complex remains stable for 1 hour, enabling rapid, stable, and highly sensitive detection of protein content. |
| Application | Coomassie Brilliant Blue G-250 dye is brownish-red in its free state and turns blue when bound to proteins. The maximum absorption peak of the dye shifts from 465 nm to 595 nm. Within a certain concentration range, the absorbance of the protein-dye complex is directly proportional to the protein content. The protein content can be quantitatively determined by measuring the change in absorbance at 595 nm. |
| Applicable Instruments | Visible Spectrophotometer/Microplate Reader |
| Number of Testable Samples | 500 Samples |
| Matching | 1 mL glass cuvette (d=10 mm)/96-well plate |
| Detection Time | 6 h (100 Samples) |
| Detection Method | Bradford |
| Spectral Parameters | 595 nm |
| Signal Response | Incremental |
| Standard | BSA |
| Reference Standards | y=3.9836x+0.8988 (R2=0.9997) |
| Standard Linear Range | 0.04-0.2 mg/mL |
| Detection Limit | 0.02 mg/mL |
| Notes | The Bradford method for determining protein concentration is not affected by most chemicals in the sample. The concentration of thioglycolic acid in the sample can reach up to 1 M, and the concentration of dithiothreitol can reach up to 5 mM. However, it is affected by slightly higher concentrations of detergents, so ensure that the SDS concentration in the sample is below 0.1%, Triton X-100 below 0.1%, and Tween 20, 60, and 80 below 0.06%. |
For research use only, not for clinical use.
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