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| Product Name | Lentivirus Concentration Solution |
| Catalog No. | RESK-0604 |
| Description | This kit contains a 5x concentration solution that enables simple and rapid concentration of all types of lentiviral particles. The procedure is straightforward: simply mix the 5x concentration solution with the lentiviral supernatant at the indicated ratio, incubate briefly, and pellet the virus using a standard centrifuge. Recovery reaches over 90%, and viral titer can be increased up to 100-fold. |
| Intended Use | Concentration of lentiviral particles from cell culture supernatants; also suitable for other retroviruses, since lentiviruses belong to the Retroviridae family. For research use only. |
| Principle / Technology | Polymer precipitation based on polyethylene glycol (PEG). PEG is highly hydrophilic and absorbs large amounts of water in solution, reducing the distance between viral particles so that they aggregate, increasing their relative concentration and allowing them to be pelleted by standard centrifugation (bioadsorption-PEG concentration). |
| Sample Type | Lentivirus-containing cell culture supernatant (also applicable to other retroviral supernatants). |
| Performance Range / Specifications | Recovery rate: over 90%. Concentration factor: up to 100-fold increase in viral titer. |
| Specificity | Suitable for all types of lentiviral particles and other retroviruses; not intended as a universal concentrator for unrelated virus classes. |
| Reaction Conditions / Protocol | 1. Collect the lentiviral supernatant and filter through a 0.45 μm membrane to remove cells and debris. Note: if filtration is used, only low-protein-binding cellulose acetate or polyethersulfone (PES) membranes may be used; nitrocellulose (NC) membranes must not be used. 2. Mix the lentiviral supernatant with the concentration solution at a 4:1 ratio (4 parts supernatant to 1 part concentration solution) and incubate at 4 °C for 2 h or overnight. Mix every 30 min at the beginning, for a total of 3 mixings. Note: extending the incubation time can increase recovery, but it also increases the proportion of adsorbed impurities; follow the recommended incubation range. Lentiviral particles remain stable for several days at 4 °C. 3. Centrifuge at 4,000 x g for 25 min at 4 °C. 4. Carefully remove the supernatant without disturbing the tube; a white pellet is usually visible (sometimes the pellet is not visible). 5. Resuspend the viral pellet in DMEM or PBS at 1/10 to 1/100 of the initial (original supernatant) volume, pipetting gently and thoroughly. 6. Determine the titer of the concentrated lentivirus, aliquot at 50 μL per tube, and store at -80 °C. Note: avoid repeated freeze-thaw cycles of frozen lentivirus stocks, as this reduces viral titer. |
| Components / Formulation | 5x concentration solution containing polyethylene glycol (PEG). |
| Storage Conditions | Store at 2-8 °C. |
| Shelf Life | 6 months |
| Package Specifications | 50 mL |
| Product Form | Liquid (5x concentration solution) |
| Concentration | 5x concentration solution; mixed with supernatant at a 1:4 ratio (concentration solution:supernatant). |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Stable for 6 months when stored at 2-8 °C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with all lentiviral particle types and other retroviruses. Filter supernatants only through low-protein-binding cellulose acetate or PES membranes; nitrocellulose membranes must not be used. Resuspend pellets in DMEM or PBS. |
| Recommended Buffer System | Resuspend concentrated viral particles in DMEM or PBS at 1/10 to 1/100 of the original supernatant volume. |
| Application Notes / Precautions | Continuous shaking during the 4 °C incubation impairs adsorption; the process requires standing time, with mixing only every 30 min at the beginning (3 times). Prolonged incubation increases recovered virus but also increases adsorbed impurities. The amount of white pellet does not reflect virus quantity: besides viral particles, the pellet contains serum proteins and small amounts of cell debris and genomic DNA; it cannot remove impurity proteins such as BSA from serum. To purify further, resuspend the virus and centrifuge at 12,000 x g for 3-5 min at 4 °C, then collect the supernatant as the concentrated virus. Whether a pellet is visible depends on the total amount of virus in the starting material. Avoid repeated freeze-thaw cycles of the concentrated virus, as they reduce titer. |
For research use only, not for clinical use.
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