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| Product Name | LB Freezing Buffer, Sterile |
| Catalog No. | RERE-0398 |
| Description | Long-lived bacterial working collections depend on a freezing matrix that protects membranes through the freeze-thaw cycle, and this buffer answers with LB medium fortified with ammonium sulfate and glycerol. Glycerol moderates ice crystal growth while the nutrient base keeps cells viable as they pass into and out of the freezer. Filter-sterilised and stored chilled, it accepts pelleted or suspended bacteria straight into cryovials. |
| Intended Use | Preparation of bacterial strains for frozen storage in glycerol-based cryopreservation workflows. |
| Principle / Technology | Glycerol lowers freezing injury by controlling ice formation while the LB base preserves viability. |
| Specificity | Composed of LB medium, ammonium sulfate and glycerol; filter-sterilised; sterile solution. |
| Reaction Conditions / Protocol | Suspend the bacterial pellet in the buffer and aliquot into cryovials for freezing. |
| Storage Conditions | 2-8°C; 6 months. |
| Package Specifications | 100 mL |
| Key Features | Glycerol-protected freezing matrix; sterile filtered; supports long-term strain banking. |
For research use only, not for clinical use.
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