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| Product Name | Lactate Dehydrogenase (LDH) Activity Assay Kit (Colorimetric) |
| Catalog No. | ETR-HMM-0073 |
| Description | A colorimetric assay kit for the quantitative measurement of lactate dehydrogenase activity in biological fluids, cell lysates, and tissue homogenates. LDH is a cytoplasmic enzyme released upon cell membrane damage, making it useful for cytotoxicity assessment. |
| Intended Use | Quantitative measurement of LDH activity in serum, plasma, cell culture supernatant, and tissue extracts for cytotoxicity studies and tissue damage assessment. |
| Principle / Technology | LDH catalyzes the conversion of lactate to pyruvate with concomitant reduction of NAD+ to NADH; the generated NADH reduces a tetrazolium salt to a colored formazan product with absorbance at 490–500 nm. |
| Detection Method | Colorimetric detection at 490–500 nm using a microplate reader or spectrophotometer |
| Sample Type | Cell culture supernatant, serum, plasma, tissue homogenates, and cell lysates |
| Performance Range / Specifications | Linear detection range: 0.1–250 mU/mL LDH activity; reaction time: 30 minutes at 37°C |
| Sensitivity / LOD | ≤0.05 mU/mL minimum detectable LDH activity |
| Specificity | Specific for LDH activity; no interference from other dehydrogenases at physiological concentrations when the supplied substrate mixture is used |
| Reaction Conditions / Protocol | Add 50 μL of sample to 50 μL of reaction mixture in a 96-well plate, incubate at 37°C for 30 minutes protected from light, add 50 μL of stop solution, and measure absorbance at 490 nm |
| Components / Formulation | Lactate substrate solution, NAD+ cofactor, tetrazolium salt (WST), diaphorase enzyme, LDH positive control, assay buffer, stop solution, 96-well plate protocol |
| Storage Conditions | -20°C for enzyme components; assay buffer and substrate at 2–8°C; protect from light |
| Shelf Life | 12 months from date of manufacture |
| Package Specifications | 500 tests in 96-well plate format |
| Product Form | Liquid reagent components requiring mixing before use |
| Quality Control | Each lot validated with purified LDH enzyme standard; linearity R² >0.99; intra-assay CV below 5% |
| Key Features | Simple colorimetric readout compatible with standard microplate readers; high sensitivity enables detection of low-level cytotoxicity; water-soluble formazan product eliminates solubilization steps |
For research use only, not for clinical use.
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