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| Product Name | Lactate Assay Kit (Fluorometric, LDH-Coupled) |
| Catalog No. | CMTR-HMM-0071 |
| Description | Fluorometric L-lactate quantification kit based on lactate dehydrogenase-catalyzed oxidation of L-lactate to pyruvate with NAD+ reduction to NADH. Enzyme cycling amplification generates a stable fluorescent signal proportional to lactate concentration. Enables sensitive detection for metabolic flux analysis between glycolysis and oxidative phosphorylation pathways. |
| Intended Use | L-lactate quantification for glycolytic rate measurement, cancer cell metabolic profiling (Warburg effect), cell culture monitoring, and hypoxia/ischemia metabolism research. |
| Principle / Technology | LDH oxidizes L-lactate to pyruvate with NAD+ to NADH; NADH drives fluorogenic probe reduction via enzyme cycling (Ex/Em 530/590 nm); stereospecific for L-lactate |
| Detection Method | Fluorometric; Ex/Em 530/590 nm; colorimetric backup at 570 nm |
| Sample Type | Cell culture supernatant, lysates, tissue homogenates, serum, plasma; 2-20 µL per assay |
| Sensitivity / LOD | Detection range 0.1-20 µM in assay; LOD 0.05 µM L-lactate |
| Specificity | Specific for L-lactate; no cross-reactivity with D-lactate or pyruvate; LDH stereospecific |
| Reaction Conditions / Protocol | Add sample and LDH/NAD+/probe reaction mix; incubate 30 min at RT; read fluorescence |
| Components / Formulation | LDH, NAD+, fluorogenic probe, enzyme amplifier, L-lactate standard (1.0 mM), assay buffer |
| Storage Conditions | Store at -20 °C; protect probe from light |
| Shelf Life | 12 months from manufacture date |
| Package Specifications | 100 assays, 500 assays in 96-well format |
| Product Form | Lyophilized enzymes; liquid buffers and probe |
| Key Features | 50× more sensitive than colorimetric NADH endpoint; LDH stereospecificity; minimal pyruvate interference; enzyme cycling extends linear range |
| Purity | LDH >400 U/mg; L-lactate standard >99% pure, enantiomeric excess >99% |
| Concentration | As specified per kit format; probe stock solutions in DMSO as specified |
| Activity / Unit Definition | Standard curve linearity R² ≥0.99 per lot; dynamic range as specified |
| Molecular Weight | Varies by probe and buffer component |
| Source / Origin | Synthetic fluorescent probes; recombinant enzymes; purified metabolite standards |
| pH Range / Optimal pH | pH 7.2–7.4 for cell-based assays; pH varies for biochemical metabolite detection |
| Shipping Conditions | Cold pack -20 °C; probe light-sensitive |
| Expiration Date / Stability | 12 months at -20 °C; reconstituted enzymes 1 week at 4 °C |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with fluorescence microplate readers, luminometers, and spectrophotometers |
| Recommended Buffer System | Assay-specific buffer optimized per product protocol |
| Application Notes / Precautions | Normalize signal to cell number or protein content; avoid probe photobleaching; include appropriate antioxidant controls for ROS assays |
| Batch-to-Batch Consistency | Standard curve parameters within ±15% of reference lot |
For research use only, not for clinical use.
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