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| Product Name | Hydroxyproline (HYP) Content Assay Kit, 50T/48S |
| Catalog No. | AACAK-YJL-0015 |
| Detection Method | Visible spectrophotometry, Spectrophotometer |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Hydroxyproline (HYP) is a non-standard amino acid almost exclusively found in collagen and, to a lesser extent, elastin. Its measurement serves as a specific and quantitative biomarker for total collagen content in tissues. Assessing HYP levels is fundamental in research on fibrosis (liver, lung, kidney), connective tissue diseases, wound healing, bone turnover, and tumor metastasis. This kit employs a classic chloramine-T oxidation method followed by reaction with Ehrlich's reagent to produce a characteristic chromogen. |
| Product Application | • Quantification of collagen deposition in models of tissue fibrosis. • Research on bone remodeling, osteoporosis, and osteoarthritis. • Assessment of wound healing and scar formation. • Studies on extracellular matrix (ECM) remodeling in cancer and cardiovascular diseases. |
| Product Advantages | • Collagen-Specific Marker: Provides a direct chemical measurement of hydroxyproline, offering a reliable proxy for total collagen content across various tissue types. • Classic, Established Methodology: Based on the well-validated chloramine-T/Ehrlich's reagent protocol, ensuring robust and reproducible results widely accepted in the field. • Includes Acid Hydrolysis Guidance: Provides a detailed protocol for optimal acid hydrolysis of tissue samples, a critical step for releasing protein-bound hydroxyproline. • Sensitive Detection: Capable of detecting hydroxyproline in the low microgram range, suitable for samples with limited collagen content. |
For research use only, not for clinical use.
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