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| Product Name | Human, Mouse and Rat rRNA and ITS/ETS Depletion Kit 2.0 (RNase H method) |
| Catalog No. | REKI-0099 |
| Description | An RNase H digestion kit that removes ribosomal RNA together with the 45S ITS/ETS precursor regions from human, mouse and rat total RNA, preserving mRNA and non-coding RNA for sequencing. Dedicated probes cover the 45S ITS/ETS sequences whose share rises sharply in degraded samples, so even poor-quality FFPE RNA yields a markedly higher fraction of usable reads. The kit suits both intact and partially degraded total RNA and connects to RNA library kits from any supplier. |
| Intended Use | rRNA and ITS/ETS removal from human, mouse and rat total RNA before RNA-seq library construction; rescue of low-quality FFPE RNA datasets. |
| Principle / Technology | Species-specific DNA probes hybridise rRNA and ITS/ETS targets, which RNase H then digests, leaving non-target RNA intact. |
| Sample Type | Human, mouse and rat total RNA including FFPE-derived RNA |
| Performance Range / Specifications | Low rRNA residual rate; effective on intact and partially degraded RNA; higher usable-read fraction on FFPE samples. |
| Specificity | Covers human, mouse and rat rRNA plus 45S ITS/ETS regions. |
| Reaction Conditions / Protocol | Hybridise probes to total RNA, digest targets with RNase H and clean the depleted RNA for library construction. |
| Components / Formulation | Probe mix and RNase H depletion reagents. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 12 T, 24 T, 96 T |
| Product Form | Boxed probe and enzyme modules |
| Key Features | ITS/ETS coverage for degraded samples; supplier-agnostic downstream compatibility. |
| Compatibility | RNA library kits from any supplier |
| Readouts / Downstream Assays | Depleted RNA for transcriptome sequencing |
For research use only, not for clinical use.
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