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| Product Name | Human CD4 Nanobeads |
| Catalog No. | RESK-0457 |
| Description | Human CD4 Nanobeads can be used for the isolation of human cells based on CD4 antigen expression. CD4 is expressed on all T cells and is associated with the T cell receptor. This product consists of a CD4 monoclonal antibody conjugated to nanoscale magnetic beads, enabling the sorting of CD4-positive T cells. The human CD4 sorting nanobeads bind directly to the cells. After magnetic labeling, the cell-bead suspension is loaded onto an MS column or LS column, or another suitable separation column, placed in the separation magnet of a mainstream magnetic separation system. After magnetic separation, the cells retained in the column are the positively selected target cells and can be used for downstream research; the flow-through can also be collected as the negatively selected cells without CD4 expression. |
| Intended Use | Isolation of human cells based on CD4 antigen expression, enabling the sorting of CD4-positive T cells. |
| Principle / Technology | The CD4 monoclonal antibody conjugated to the nanobeads binds directly to CD4-expressing cells, magnetically labeling them. During column-based magnetic separation in a separation magnet, labeled cells are retained in the column (positive selection) while unlabeled cells flow through (negative selection fraction). |
| Sample Type | Human cells (e.g., T cells from PBMC samples) |
| Performance Range / Specifications | Matrix: dextran nanobeads Ligand: Anti-Human CD4 antibody Particle size: 50-100 nm Separation capacity: up to 5×10^8 cells/mL beads Sorting capacity by package size: 10 T: up to 1×10^8 cells 50 T: up to 5×10^8 cells 100 T: up to 1×10^9 cells Storage buffer: 1X PBS (pH 7.2-7.4) containing 2 mM EDTA and 0.5% BSA, without Ca2+ |
| Specificity | The conjugated anti-human CD4 monoclonal antibody specifically binds the CD4 antigen expressed on T cells. |
| Reaction Conditions / Protocol | 1) Incubate the human cells with Human CD4 Nanobeads so that the nanobeads bind directly to CD4-expressing cells. 2) Load the cell-bead suspension onto an MS column or LS column, or another suitable separation column, placed in the separation magnet of a magnetic separation system. 3) After magnetic separation, the cells retained in the column are the positively selected CD4-expressing cells; the flow-through can be collected as the negatively selected cells without CD4 expression. |
| Components / Formulation | Human CD4 Nanobeads (dextran magnetic nanobeads conjugated with an anti-human CD4 monoclonal antibody) supplied in storage buffer: 1X PBS (pH 7.2-7.4) containing 2 mM EDTA and 0.5% BSA, without Ca2+ |
| Storage Conditions | Store at 2-8 °C protected from light. |
| Shelf Life | 6 months |
| Package Specifications | 10 T 50 T 100 T |
| Product Form | Dextran magnetic nanobead suspension (50-100 nm) |
| Quality Control | When CD4 T cells are sorted from T cells of PBMC samples with this product, the purity, recovery, and viability of the sorted cells are comparable to those obtained with mainstream commercial magnetic beads. |
| Purity | High purity of sorted cells (comparable to mainstream commercial magnetic beads) |
| Source / Origin | Anti-human CD4 monoclonal antibody conjugated to dextran magnetic nanobeads |
| pH Range / Optimal pH | pH 7.2-7.4 (storage buffer) |
| Expiration Date / Stability | Valid for 6 months when stored at 2-8 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with MS columns, LS columns, and other suitable separation columns used with the separation magnets of mainstream magnetic separation systems. For optimal separation results, this product can be used together with MS columns, a single-channel separation magnet, or a universal sorting stand from Alta DiagnoTech. |
| Recommended Buffer System | Storage buffer: 1X PBS (pH 7.2-7.4) containing 2 mM EDTA and 0.5% BSA, without Ca2+ |
| Application Notes / Precautions | High safety: bead particle size (50-100 nm) is equivalent to mainstream commercial products, with low toxicity. Excellent performance: high purity, high activity, and high recovery. Convenient operation: biodegradable matrix with no need for additional bead removal. High quality: high stability and high compatibility. For optimal separation results, this product can be used together with MS columns, a single-channel separation magnet, or a universal sorting stand from Alta DiagnoTech. |
| Recognition Site | Human CD4 antigen |
For research use only, not for clinical use.
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