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| Product Name | High-ROX qPCR Library Quantification Kit for Illumina Libraries |
| Catalog No. | NG-QCY-0044 |
| Description | A dye-based qPCR kit for absolute quantification of Illumina-compatible sequencing libraries. Library concentration is calculated from a standard curve generated with supplied DNA standards, while High ROX is premixed for compatible instruments. |
| Intended Use | absolute concentration measurement of sequencing libraries and assessment of library contamination in the laboratory environment |
| Principle / Technology | Standard-curve-based absolute quantification using hot-start dye qPCR |
| Detection Method | Real-time fluorescence qPCR |
| Sample Type | Illumina-compatible sequencing libraries |
| Performance Range / Specifications | 500 reactions at 20 μL per reaction; inter-batch standard CV below 5% |
| Sensitivity / LOD | High-sensitivity qPCR detection across the supplied standard range |
| Specificity | Library-specific amplification depends on compatibility between the kit primers and library adapter sequences. |
| Reaction Conditions / Protocol | Prepare standards and diluted libraries, run dye-based qPCR and calculate absolute concentration from the standard curve. |
| Components / Formulation | High-ROX hot-start dye qPCR master mix, library-quantification primers and DNA standards |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 500 reactions |
| Product Form | Multi-component fluorescence quantification kit |
| Quality Control | Standards and detection reagents are functionally tested for quantitative performance. |
| Key Features | Complete quantification system; premixed High ROX; high amplification efficiency; broad GC tolerance; batch CV below 5%. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Illumina-compatible libraries and real-time PCR instruments requiring High ROX passive reference. |
| Batch-to-Batch Consistency | Standard quantification performance is controlled with an inter-batch CV below 5%. |
For research use only, not for clinical use.
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