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| Product Name | High-Purity Exosome Isolation Kit (for Serum/Plasma) |
| Catalog No. | RESK-0619 |
| Description | Exosomes are small vesicles of approximately 30-150 nm in diameter secreted by living cells, with a typical lipid bilayer structure, found in cell culture supernatant, serum, plasma, saliva, urine, amniotic fluid, and other biological fluids. They carry proteins, lipids, DNA, and RNA, play important roles in intercellular material and information transfer, and are promising early diagnostic biomarkers for many diseases. With optimized components for serum/plasma samples, this kit uses a precipitation method to rapidly obtain large quantities of intact exosome particles, followed by filter-based purification to deliver high-purity exosomes. Compared with the standard precipitation-only versions, the added contaminant-protein removal step yields exosomes of higher purity with less impact on downstream experiments, making the kit recommended for proteomics applications. |
| Intended Use | Rapid isolation and purification of high-purity exosomes from serum/plasma. Isolated exosomes are suitable for downstream cell co-culture, electron microscopy, Western Blot, quantitative PCR (qPCR), and high-throughput sequencing. For serum/plasma samples only; not applicable to other body fluids or cell culture supernatant. |
| Principle / Technology | Precipitation-based exosome enrichment combined with contaminant-protein removal: Solution A removes impurity proteins, Solution B precipitates exosomes, and the Exosome Purification Filter provides final purification of intact exosome particles. |
| Detection Method | Downstream analysis: electron microscopy, Western Blot, qPCR, high-throughput sequencing, cell co-culture. |
| Sample Type | Serum or plasma; 500 uL per reaction (make up to 500 uL with 1x PBS if the sample volume is insufficient). |
| Performance Range / Specifications | Rapid and efficient recovery of high-purity exosome particles from serum/plasma. Exosome identity is typically confirmed by three criteria: morphological observation by electron microscopy, particle size measurement (30-150 nm), and protein marker detection (CD9, CD81, CD63, Alix, TSG101) by Western Blot. Advantages over ultracentrifugation: simple operation, no additional instruments required, time saving, and compatible with small sample volumes. |
| Specificity | Specific for serum/plasma samples; not suitable for exosome extraction from other body fluids or cell culture supernatant. |
| Reaction Conditions / Protocol | 1. Sample pretreatment - Pre-cool the centrifuge at 4 °C for 10 min. - Thaw frozen samples at 2-8 °C; keep fresh samples at 2-8 °C after collection. - Centrifuge serum/plasma at 10,000 x g for 10 min at 4 °C, then aliquot at 500 uL per tube; make up to 500 uL with 1x PBS if needed. 2. Contaminant protein removal - Add 400 uL of pre-cooled Solution A to the 500 uL sample, cap tightly, and vortex thoroughly for 30 s. - Centrifuge at 10,000 x g for 20 min at 4 °C and transfer the supernatant to a new 1.5 mL tube. 3. Exosome precipitation - Add 120 uL of Solution B, cap tightly, vortex for 2 min, and stand at room temperature for 5 min. - Centrifuge at 10,000 x g for 15 min at 4 °C and discard the supernatant. - Centrifuge again at 10,000 x g for 2 min at 4 °C, remove the supernatant as completely as possible, and stand the open tube at room temperature for 10 min. - Resuspend the pellet in 200 uL of 1x PBS by repeated pipetting or vortexing (or soak overnight at 4 °C). - Centrifuge at 10,000 x g for 5 min at 4 °C and keep the supernatant, which is enriched in exosome particles. 4. Exosome purification - Transfer the crude exosomes into the upper chamber of the Exosome Purification Filter and centrifuge at 3,000 x g for 10 min at 4 °C (extend as needed until all liquid collects at the bottom); the liquid at the bottom contains the purified exosomes. - Aliquot purified exosomes at 50-100 uL and store at -80 °C. |
| Components / Formulation | Solution A: 6 mL (10 T) / 12 mL (20 T), store at 2-8 °C Solution B: 2 mL (10 T) / 5 mL (20 T), store at room temperature Exosome Purification Filter: 10 tubes (10 T) / 20 tubes (20 T), store at room temperature |
| Storage Conditions | Store components at room temperature or 2-8 °C according to the individual component requirements; mix thoroughly before use. |
| Shelf Life | 12 months (stored according to individual component requirements). |
| Package Specifications | 10 T, 20 T |
| Shipping Conditions | Shipped at room temperature. |
| Regulatory / Compliance | For research use only. |
| Recommended Buffer System | 1x PBS (for sample make-up volume and exosome resuspension). |
| Application Notes / Precautions | Mix the exosome extraction reagent (Solution A) thoroughly before use. The Exosome Purification Filter is single-use and cannot be reused. For serum/plasma samples only; not applicable to other body fluids or cell culture supernatant. For further purification, antibody-coated magnetic beads can be used for affinity purification. Serum/plasma-derived exosomes contain abundant contaminating proteins that may clog the filter membrane; resuspend the pellet thoroughly by repeated pipetting before loading onto the purification filter. Storage of extracted exosomes: for short-term use, store at 2-8 °C for up to one week; for long-term storage, keep at -80 °C, avoid repeated freeze-thaw cycles, and preferably use low protein-binding tubes. |
For research use only, not for clinical use.
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