High-Performance Streptavidin Agarose Affinity Resin
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High-Performance Streptavidin Agarose Affinity Resin

Cat.No: IGM-HMM-0083 Datasheet

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Product Name High-Performance Streptavidin Agarose Affinity Resin
Catalog No. IGM-HMM-0083
Description A high-resolution affinity resin based on 6% cross-linked agarose with immobilized streptavidin for selective capture and purification of biotinylated biomolecules.
Intended Use Capture of biotinylated nucleic acids, antibodies and proteins in pyrosequencing, nucleic acid hybridization capture and protein purification workflows.
Principle / Technology Immobilized streptavidin provides high-affinity binding to biotinylated ligands on a small-particle agarose matrix that supports high-resolution separations.
Performance Range / Specifications Matrix: 6% agarose. Ligand: streptavidin. Binding capacity: 300 nmol biotin/mL. Median particle size: approximately 34 µm. Recommended flow rate: below 150 cm/h. Pressure tolerance: 0.3 MPa.
Specificity Selective capture of biotin and biotinylated biomolecules.
Components / Formulation Streptavidin immobilized on 6% cross-linked agarose; supplied in 20% ethanol.
Storage Conditions Store at 2-8°C in 20% ethanol. Do not freeze.
Shelf Life 3 years when stored as directed.
Package Specifications 5 mL; 25 mL; 100 mL; 1 L.
Product Form Agarose affinity chromatography resin suspension.
Key Features High resolution; low nonspecific adsorption; broad compatibility with biotinylated ligands; high biotin-binding capacity.
pH Range / Optimal pH Operating pH 4-9; cleaning-in-place stability pH 3-10.
Shipping Conditions Ship at 25°C or below. Do not freeze.
Compatibility Gravity-flow and column chromatography formats for biotin-affinity capture.
Recommended Buffer System Supplied and stored in 20% ethanol; equilibrate with an application-appropriate neutral buffer before use.
Application Notes / Precautions Do not freeze. Maintain flow below the recommended limit and avoid exceeding 0.3 MPa operating pressure.

For research use only, not for clinical use.

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