High-Fidelity NGS Library Amplification Mix

Name: High-Fidelity NGS Library Amplification Mix
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Cat.No: NG-QCY-0014 Datasheet

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Product Details Related Products

Product Name	High-Fidelity NGS Library Amplification Mix
Catalog No.	NG-QCY-0014
Description	A 2× high-fidelity PCR premix for amplification and enrichment of next-generation sequencing libraries.
Intended Use	Amplification of whole-genome, targeted, amplicon and RNA sequencing libraries.
Principle / Technology	An engineered high-fidelity DNA polymerase and optimized buffer provide uniform, low-bias library amplification with a low mismatch rate.
Detection Method	Downstream library quality control and next-generation sequencing.
Sample Type	Prepared DNA or RNA-derived sequencing libraries.
Performance Range / Specifications	24 or 96 reactions; polymerase fidelity reported at approximately 82-fold higher than Taq DNA polymerase.
Specificity	High-fidelity polymerase and optimized buffer preserve library composition and improve mutation-call accuracy.
Reaction Conditions / Protocol	Combine the 2× premix with a prepared library and platform-compatible amplification primers, then run the validated library PCR program.
Components / Formulation	2× high-fidelity library amplification premix containing DNA polymerase, dNTPs, magnesium and optimized buffer.
Storage Conditions	Store at −30 to −15°C.
Package Specifications	24 reactions; 96 reactions.
Product Form	2× high-fidelity library amplification master mix.
Quality Control	Library yield, amplification uniformity, sequencing uniformity and polymerase fidelity are evaluated.
Key Features	High fidelity; uniform amplification; low sequence bias; ready-to-use 2× format; suitable for multiple library types.
Shipping Conditions	Ship at or below 0°C.
Compatibility	Available for Illumina and single-index MGI library workflows.

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For research use only, not for clinical use.