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| Product Name | High-Fidelity 2x Library Amplification Premix (fast extension, stabilised activity) |
| Catalog No. | REKI-0090 |
| Description | A 2x ready-to-use amplification premix built around a high-fidelity polymerase offering six-fold fidelity over standard polymerase and extension at 15 sec per kilobase. The formulation pairs the enzyme with dNTPs and a buffer optimised for sequencing-library amplification; a dedicated stabiliser keeps activity intact through repeated freeze-thaw cycles, so partially used aliquots stay reliable. Only primers and template are added, cutting handling steps and improving inter-run reproducibility. |
| Intended Use | Amplification of DNA sequencing libraries in short-read workflows. |
| Principle / Technology | High-fidelity polymerase copies library molecules with proofreading activity, enriched in a library-optimised buffer with protective agents. |
| Sample Type | Adapter-ligated DNA libraries |
| Performance Range / Specifications | Fidelity six-fold that of standard polymerase; 15 sec/kb extension; activity stable after repeated freeze-thaw. |
| Specificity | Validated alongside established fragmentation and ligation modules in complete library workflows. |
| Reaction Conditions / Protocol | Add primers and library template to the premix and amplify. |
| Components / Formulation | High-fidelity DNA polymerase, dNTPs, optimised buffer, protective agent. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 18 months |
| Package Specifications | 24 T, 96 T |
| Product Form | 2x premix solution |
| Key Features | Fast extension; freeze-thaw stable; minimal handling setup. |
| Concentration | 2x |
| Compatibility | Short-read sequencing library workflows |
| Readouts / Downstream Assays | Enriched sequencing libraries |
For research use only, not for clinical use.
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