| Product Name | Hexokinase Activity Assay Reagent |
| Catalog No. | CMTR-HMM-0002 |
| Description | Hexokinase (HK) is present in many organisms, including bacteria, plants, and mammals, and plays a crucial role in glucose metabolism. Hexokinase phosphorylates glucose to form glucose-6-phosphate, which is used in glycolysis. Hexokinase has four subtypes (HK-I, II, III, and IV). HK-I, HK-II, and HK-III have lower Km values, while HK-IV has a Km value up to 100 times higher. |
| Application | The product employs a simple, sensitive, and rapid procedure capable of detecting hexokinase activity below 0.1 mU/well. |
| Principle | Glucose is converted to 6-phosphoglucose by hexokinase. Then, 6-phosphoglucose is oxidized by 6-phosphoglucose dehydrogenase to form NADH. NADH reduces the colorless probe to a colored product with strong absorbance at 450 nm. |
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h |
| Sample Type | Adherent cells, Serum, Suspension cells, Tissue, Tissue extracts |
| Detection Limit | 0.1 mU/well |
| Procedure | 1. Add samples and standards to wells. 2. Add reaction mixture and incubate for 20-60 minutes. 3. Analyze repeatedly during incubation using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2025 Alta DiagenoTech. All rights reserved.
