| Product Name | Glycosylated Hemoglobin (GHb) Content Assay Reagent (Microplate Reader) |
| Catalog No. | PTR-HMM-0046 |
| Description | Glycated hemoglobin (GHb) is formed when hemoglobin in red blood cells reacts with sugars (primarily glucose) in serum. Glycated hemoglobin is composed of HbA1a, HbA1b, and HbA1c, with HbA1c accounting for approximately 70% of the total and having a relatively stable structure. The non-enzymatic reaction of glycated hemoglobin is characterized by being continuous, slow, and irreversible. Its concentration is determined by past rather than current blood glucose levels and is not influenced by factors such as fasting status, insulin injection, or the use of antidiabetic medications prior to testing. It is commonly used for monitoring diabetes control indicators. |
| Application | Under acidic conditions, the sugar on glycated hemoglobin is heated, dehydrated, and condensed to form 5-hydroxymethylfurfural (5-HMF). 5-HMF reacts with TBA (Thiobarbituric Acid, TBA) to form a colored substance, which has a characteristic absorption peak at 443 nm. The content of glycated hemoglobin can be quantitatively detected by measuring the change in absorbance. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 96 Samples |
| Matching | 96-well plate |
| Detection Time | 5 h (96 Samples) |
| Detection Method | TBA |
| Spectral Parameters | 443 nm |
| Signal Response | Incremental |
| Notes | When adding reagent three, mix thoroughly while adding. After adding part of reagent three, the reaction solution may solidify. Stir thoroughly before centrifuging. If the total hemoglobin measurement absorbance ΔA total is greater than 1.0, it is recommended to dilute the sample with distilled water before testing and make corresponding adjustments in the calculation. If the total hemoglobin measurement absorbance value ΔA total is less than 0.02, it is recommended to increase the sample volume during sample preparation, re-extract the sample, and then perform the measurement, making corresponding adjustments during calculation; If the glycated hemoglobin measurement absorbance value ΔA is greater than 1.0, it is recommended to dilute the sample with distilled water before measurement, making corresponding adjustments during calculation; If the absorbance value ΔA measured for glycated hemoglobin is less than 0.02, it is recommended to increase the sample volume during sample preparation, re-extract the sample, and then perform the measurement again, making corresponding adjustments to the calculation; |
For research use only, not for clinical use.
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