| Product Name | Glutathione Peroxidase (GPX) Activity Assay Reagent (Visible Spectrophotometer) |
| Catalog No. | ETR-HMM-0021 |
| Description | Glutathione peroxidase (GPX) is a widely distributed and important peroxidase in the body. GPX catalyzes the conversion of reduced glutathione (GSH) into oxidized glutathione (GSSG), thereby reducing toxic hydrogen peroxide into non-toxic hydroxyl compounds. This protects biological membranes from ROS damage and maintains normal cellular function. Additionally, it has protective effects on the liver, enhances immune function, antagonizes the harmful effects of metal ions on the body, and increases radiation resistance. |
| Application | Glutathione peroxidase catalyzes the oxidation of GSH by H₂O₂ to form GSSG, GSH reacts with 5,5'-dithiobis-(2-nitrobenzoic acid) to form yellow 2-nitro-5-mercaptobenzoic acid, which has a characteristic absorption peak at 412 nm. The activity of glutathione peroxidase can be characterized by the rate of decrease in absorbance. |
| Applicable Instruments | Visible Spectrophotometer |
| Number of Testable Samples | 25 Samples |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Detection Time | 4 h (25 Samples) |
| Detection Method | DTNB method |
| Spectral Parameters | 412 nm |
| Signal Response | Decremental |
| Notes | If the measured absorbance value exceeds 1.0, it is recommended to dilute the crude enzyme solution with the extraction solution before measurement and adjust the calculations accordingly; Measure the absorbance immediately after color development to avoid prolonged detection time affecting color development and causing measurement errors. |
For research use only, not for clinical use.
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