| Product Name | Glutamate Cysteine Ligase (GCL) Activity Assay Reagent (Visible Spectrophotometer) |
| Catalog No. | ETR-HMM-0015 |
| Description | Gamma-glutamyl cysteine ligase (GCL), also known as glutamyl-L-cysteine ligase, is the rate-limiting enzyme in the glutathione (GSH) biosynthetic pathway, and glutathione exerts a feedback inhibitory effect on it. The expression of the γ-glutamylcysteine ligase gene is regulated by various factors, including oxidants, antioxidants, growth factors, and inflammatory factors. Its activity significantly influences glutathione levels, the GSH/GSSG ratio, and glutathione biosynthetic capacity. |
| Application | γ-Glutamylcysteine ligase catalyzes the synthesis of γ-glutamylcysteine from glutamate and cysteine, while dephosphorylating ATP to form inorganic phosphate. The activity of γ-glutamylcysteine ligase can be characterized by measuring the rate of increase in inorganic phosphate using the molybdenum blue method. |
| Applicable Instruments | Visible Spectrophotometer |
| Number of Testable Samples | 25 Samples |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Detection Time | 4 h (25 Samples) |
| Materials to Bring | Concentrated sulfuric acid (H₂SO₄, MW = 98.078, CAS: 7664-93-9) |
| Detection Method | Molybdenum blue method |
| Spectral Parameters | 660 nm |
| Signal Response | Incremental |
| Notes | The preparation of crude enzyme solution should be performed on ice, and enzyme activity measurements should be completed as soon as possible to avoid inactivation affecting the results; If the A measurement exceeds 1.0, it is recommended to dilute the crude enzyme solution appropriately before testing and adjust the calculations accordingly; Reagent 3 has a short shelf life after preparation. To facilitate experimental scheduling, an additional bottle is provided as a backup, with each bottle sufficient for at least 25 sample measurements; If black insoluble particles appear during the preparation of Reagent 5, this is a normal phenomenon; simply aspirate the clear solution for use; |
For research use only, not for clinical use.
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