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| Product Name | Glutamate Cysteine Ligase (GCL) Activity Assay Reagent (Microplate Reader) |
| Catalog No. | ETR-HMM-0016 |
| Description | γ-Glutamylcysteine ligase (GCL), also known as glutamyl-L-cysteine ligase, is the rate-limiting enzyme in the glutathione (GSH) biosynthetic pathway, and glutathione exerts a feedback inhibitory effect on it. The expression of the γ-glutamylcysteine ligase gene is regulated by various factors, including oxidants, antioxidants, growth factors, and inflammatory factors. Its activity has a significant impact on glutathione content, GSH/GSSG ratio, and glutathione biosynthetic capacity. |
| Application | γ-Glutamylcysteine ligase catalyzes the synthesis of γ-glutamylcysteine from glutamic acid and cysteine, simultaneously dephosphorylating ATP to produce inorganic phosphorus. The activity of γ-glutamylcysteine ligase can be characterized by measuring the rate of increase in inorganic phosphorus using the molybdenum blue method. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 50 Samples |
| Matching | 96-well plate |
| Detection Time | 5 h (50 Samples) |
| Materials to Bring | Concentrated sulfuric acid (H₂SO₄, MW = 98.078, CAS: 7664-93-9) |
| Detection Method | Molybdenum blue method |
| Spectral Parameters | 660 nm |
| Signal Response | Incremental |
| Notes | The preparation of crude enzyme solution should be performed on ice, and enzyme activity measurement should be completed promptly to avoid inactivation affecting the results. If the A measurement exceeds 1.0, it is recommended to dilute the crude enzyme solution appropriately before measurement and adjust the calculations accordingly. Reagent 3 has a short shelf life after preparation. An additional bottle is provided as a backup for convenient experimental scheduling, with each bottle sufficient for at least 30 sample determinations. If black insoluble particles appear during the preparation of Reagent 5, this is a normal phenomenon; simply use the clear solution. |
For research use only, not for clinical use.
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