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| Product Name | Fungal rRNA Depletion Kit |
| Catalog No. | NG-QCY-0019 |
| Description | An RNase H-based depletion kit for removing ribosomal RNA from total RNA of molds, yeasts, Candida, mushrooms and other fungi. |
| Intended Use | mRNA and noncoding RNA analysis from diverse fungal species. |
| Principle / Technology | Fungal-targeted probes hybridize to rRNA, followed by RNase H digestion and DNase I treatment to retain mRNA and other noncoding RNA. |
| Detection Method | Downstream RNA-seq library preparation and sequencing. |
| Sample Type | Intact or partially degraded fungal total RNA. |
| Performance Range / Specifications | Input range 0.01–1 µg total RNA; 6, 12 or 24 reactions; approximately 1-hour workflow. |
| Sensitivity / LOD | Reported residual rRNA below 5%. |
| Specificity | Targets 5S, 5.8S, 18S, 28S, 12S and 16S fungal rRNA while preserving mRNA and noncoding RNA. |
| Reaction Conditions / Protocol | Hybridize fungal total RNA with depletion probes, digest with RNase H and DNase I, and purify the depleted RNA. |
| Components / Formulation | Fungal rRNA depletion probes, RNase H digestion reagents and DNase I treatment reagents. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 6 reactions; 12 reactions; 24 reactions. |
| Product Form | Multi-component fungal rRNA depletion kit. |
| Quality Control | Residual rRNA, fungal-species compatibility and downstream RNA-seq performance are evaluated. |
| Key Features | Broad fungal coverage; below 5% residual rRNA; approximately 1-hour workflow; compatible with degraded RNA. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for fungal mRNA and noncoding RNA sequencing workflows. |
For research use only, not for clinical use.
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