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| Product Name | Full-Length cDNA Library Prep Kit for Long-Read Nanopore Platforms (Template-Switching) |
| Catalog No. | REKI-0106 |
| Description | A dedicated kit for building full-length cDNA libraries read on a long-read nanopore platform. Reverse transcription starts from an oligo(dT) primer and exploits the terminal transferase activity of the reverse transcriptase to append a defined sequence at the cDNA 3-prime end; PCR then uses that anchor together with the oligo(dT) end to amplify complete cDNA molecules. Libraries preserve whole-transcript structure, so transcript isoforms, fusion transcripts and condition-specific expression changes can be resolved across human, mouse, zebrafish and yeast material. |
| Intended Use | Full-length transcriptome sequencing, differential transcriptome analysis under stress or developmental time courses, comparative transcriptomics across closely related species, fusion gene identification in oncology research, quantitative transcript profiling. |
| Principle / Technology | Oligo(dT)-primed reverse transcription with template switching appends a universal anchor at the cDNA 3-prime end; full-length cDNA is PCR amplified with the anchor and oligo(dT) primers before library construction. |
| Sample Type | Human, mouse, zebrafish, yeast total RNA |
| Performance Range / Specifications | Captures complete transcript structures from polyadenylated RNA; suited to structural and differential transcriptome analysis. |
| Reaction Conditions / Protocol | Reverse transcribe with oligo(dT) primer and template switching, amplify full-length cDNA, then construct the sequencing library. |
| Components / Formulation | Oligo(dT) primer, template-switching reverse transcription reagent, PCR reagents, library construction buffers. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 8 T, 24 T, 96 T |
| Product Form | Liquid reagents |
| Key Features | Template-switching chemistry preserves full-length information; dedicated compatibility with the designated nanopore instrument. |
| Compatibility | CycloneSEQ nanopore sequencing platform (instrument-specific reagent) |
| Readouts / Downstream Assays | Full-length transcript isoforms, fusion transcripts and quantitative transcriptome data |
For research use only, not for clinical use.
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