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| Product Name | Flow Cytometry Sample Diluent, Isotonic Saline with BSA, Azide-Free |
| Catalog No. | ATRD-0024 |
| Description | Azide-free isotonic diluent formulated for flow cytometry sample preparation and sheath fluid applications in cell sorters and analytical cytometers. Contains 0.5% bovine serum albumin (BSA) as a protein stabilizer to reduce nonspecific antibody binding and maintain cell viability during extended acquisition runs. The azide-free composition eliminates metabolic interference with live cell experiments and is compatible with functional assays including calcium flux, proliferation, and cytotoxicity measurements performed on sorted cells. |
| Intended Use | Dilution of whole blood, bone marrow, and single-cell suspensions for flow cytometric immunophenotyping; sheath fluid for cell sorting applications requiring post-sort cell culture or functional assays. |
| Principle / Technology | Isotonic saline (290 ± 10 mOsm/kg) maintains cell volume homeostasis; BSA blocks Fc receptor-mediated nonspecific antibody binding; EDTA chelates divalent cations to prevent cell aggregation. |
| Detection Method | Dilute cell suspensions to desired concentration (typically 1-10 × 10^6 cells/mL); filter through 40 µm strainer before acquisition; maintain samples at 2-8 °C during extended runs. |
| Sample Type | Whole blood, peripheral blood mononuclear cells (PBMCs), bone marrow aspirates, splenocyte suspensions, lymph node cell preparations, cultured cell lines. |
| Performance Range / Specifications | Maintains >95% cell viability for up to 4 hours at 4 °C or 2 hours at room temperature; background fluorescence equivalent to PBS with 0.5% BSA. |
| Sensitivity / LOD | Autofluorescence background within 1% of PBS baseline across 405 nm, 488 nm, 561 nm, and 640 nm excitation wavelengths. |
| Specificity | Azide-free and preservative-free to avoid metabolic interference; EDTA inhibits cell aggregation without affecting surface antigen expression. |
| Reaction Conditions / Protocol | Dilute samples as needed; incubate at 2-8 °C during staining and acquisition; use within 4 hours of dilution for viability-dependent assays. |
| Components / Formulation | Sodium chloride 0.9% w/v, BSA (Fraction V) 0.5% w/v, EDTA disodium 2 mM, HEPES buffer 10 mM, deionized water. |
| Storage Conditions | Store at 2-8 °C; protect from light; do not freeze. |
| Shelf Life | 12 months from date of manufacture when stored as directed. |
| Package Specifications | 100 mL, 500 mL, 1 L sterile bottles. |
| Product Form | Clear, colorless to pale yellow liquid; sterile-filtered 0.22 µm. |
| Quality Control | Each lot tested for sterility, endotoxin <0.1 EU/mL, osmolarity 290 ± 10 mOsm/kg, pH 7.4 ± 0.1, and absence of detectable azide by spectrophotometry. |
| Key Features | Azide-free and preservative-free; BSA-stabilized for reduced nonspecific binding; isotonic formulation; sterile-filtered; low endotoxin; compatible with live cell sorting and functional assays. |
| Purity | BSA Fraction V, ≥96% purity by agarose electrophoresis; sodium chloride USP grade; EDTA ≥99%. |
| Concentration | Ready-to-use 1× formulation; no dilution required. |
| Activity / Unit Definition | Protein stabilizing activity of BSA confirmed by ability to maintain antibody reactivity after 4-hour incubation. |
| Molecular Weight | BSA: ~66.5 kDa monomer; NaCl: 58.44 g/mol. |
| Source / Origin | BSA derived from bovine plasma sourced from BSE-free countries; synthetic buffer components. |
| pH Range / Optimal pH | pH 7.4 ± 0.1 at 25 °C. |
| Shipping Conditions | Cold pack (2-8 °C) recommended. |
| Expiration Date / Stability | 12 months at 2-8 °C; after opening, use within 3 months under aseptic conditions. |
| Regulatory / Compliance | For research use only; not for diagnostic procedures. BSA sourced from USDA-approved facilities. |
| Compatibility | Compatible with all commercially available fluorochrome-conjugated antibodies and tandem dyes. Do not supplement with sodium azide as it may precipitate with divalent cations in formulation. |
| Recommended Buffer System | 10 mM HEPES buffer, pH 7.4; EDTA as chelating agent. |
| Application Notes / Precautions | Pre-wet cell strainer with diluent before filtering samples. For intracellular staining, add 0.1% saponin or alternative permeabilization reagent as needed. Do not use for calcium flux assays — EDTA chelation may affect results; use calcium-containing diluent for such applications. |
| Batch-to-Batch Consistency | Osmolarity within ±5 mOsm/kg; pH within ±0.1 units; BSA concentration within ±0.05% w/v of reference lot. |
For research use only, not for clinical use.
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