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| Product Name | TMB Substrate Diluent for ELISA, Peroxidase-Compatible |
| Catalog No. | ATRD-0019 |
| Description | Ready-to-use diluent buffer optimized for TMB chromogenic substrate concentrates in HRP-based ELISA. Provides optimal pH, buffer capacity, and H₂O₂ concentration for maximum TMB sensitivity with low background. Compatible with both single-component and two-component TMB substrate systems. |
| Intended Use | Dilution of TMB substrate concentrates for HRP-based ELISA detection; working TMB substrate preparation for colorimetric immunoassays; custom ELISA development. |
| Principle / Technology | Phosphate-citrate buffer pH 4.0-4.5 with H₂O₂ co-substrate and proprietary stabilizers preventing spontaneous TMB oxidation |
| Detection Method | Mix TMB concentrate with diluent at 1:10 to 1:20 ratio; use immediately; protect from light |
| Components / Formulation | Phosphate-citrate buffer (pH 4.2), hydrogen peroxide (0.015%), stabilizers, ProClin 150 (<0.05%) |
| Storage Conditions | 2-8 °C; protect from light; do not freeze |
| Shelf Life | 24 months from manufacture date |
| Package Specifications | 100 mL, 500 mL, 1 L HDPE bottles |
| Product Form | Clear colorless liquid, ready-to-use |
| Key Features | Pre-formulated for optimal TMB kinetics; low blank (OD <0.05 at 450 nm after 30 min); working solution stable 4 hr at RT; preservative system prevents microbial growth |
| Purity | Buffer components ACS grade; H₂O₂ verified by titrimetry |
| Concentration | Ready-to-use; no dilution required |
| Activity / Unit Definition | Dilution linearity R² ≥0.99 across specified range |
| Molecular Weight | Not applicable — buffered protein solution |
| Source / Origin | Bovine serum albumin, casein, and synthetic buffer components |
| pH Range / Optimal pH | pH 4.2 ± 0.1 at 25 °C |
| Shipping Conditions | Ambient temperature; protect from heat >30 °C |
| Expiration Date / Stability | 24 months at 2-8 °C; 6 months after opening; discard if blue color develops |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with ELISA, CLIA, and immunoturbidimetric assay platforms |
| Recommended Buffer System | PBS-based with protein stabilizers and antimicrobial preservative |
| Application Notes / Precautions | Bring to room temperature before use; avoid repeated freeze-thaw; use aseptic technique for opened bottles |
| Batch-to-Batch Consistency | Dilution recovery 90–110% for reference analyte panel per lot |
For research use only, not for clinical use.
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