Whole Blood Stabilizing Diluent for Flow Cytometry

Name: Whole Blood Stabilizing Diluent for Flow Cytometry
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Cat.No: ATRD-0015 Datasheet

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Product Name	Whole Blood Stabilizing Diluent for Flow Cytometry
Catalog No.	ATRD-0015
Description	A specially formulated diluent with fixative properties for the dilution and short-term stabilization of whole blood specimens prior to immunophenotyping by flow cytometry, preserving surface antigen expression.
Intended Use	Diluting whole blood for direct immunofluorescence staining in lymphocyte subset enumeration, leukemia/lymphoma immunophenotyping, and CD4/CD8 monitoring in clinical flow cytometry laboratories.
Principle / Technology	Paraformaldehyde at low concentration partially cross-links surface proteins to stabilize antigen expression; sodium azide inhibits microbial growth and metabolic activity; BSA blocks Fc receptor binding.
Detection Method	Flow cytometric antigen expression comparison pre and post 24-hour storage, forward/side scatter gating stability
Sample Type	EDTA or heparin anticoagulated whole blood
Performance Range / Specifications	Antigen expression MFI shift <10% after 24 hours at 4°C
Sensitivity / LOD	N/A
Specificity	N/A
Reaction Conditions / Protocol	Dilute whole blood 1:2 to 1:10; mix gently with antibody cocktail; incubate per protocol; lyse RBCs before acquisition.
Components / Formulation	PBS pH 7.4, BSA 1%, sodium azide 0.1%, paraformaldehyde 0.5%, EDTA 2 mM
Storage Conditions	2–8°C, light sensitive
Shelf Life	6 months
Package Specifications	100 mL, 500 mL
Product Form	Liquid, ready to use
Quality Control	Antigen stability validation with CD3, CD4, CD8, CD19 panel, sterility, osmolarity
Key Features	Stabilizes surface antigens; ready to use; validated for lymphocyte subset analysis; compatible with lyse-wash and lyse-no-wash protocols

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For research use only, not for clinical use.