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| Product Name | Western Blot Primary Antibody Diluent, Protein-Free Formulation |
| Catalog No. | ATRD-0022 |
| Description | Protein-free, synthetic polymer-based antibody diluent eliminating antibody cross-reactivity with blocking proteins. Chemically defined environment reduces non-specific bands and is ideal for phospho-specific antibodies where milk-derived phosphatases may compromise results. Compatible with chemiluminescent and fluorescent detection systems. |
| Intended Use | Primary antibody dilution for Western blot and ELISA; phospho-specific antibody applications; biotin-streptavidin detection; quantitative immunoblotting where reproducibility is critical. |
| Principle / Technology | Synthetic polymer matrix mimics molecular crowding; non-ionic surfactants reduce hydrophobic non-specific binding; preservative-free for sensitive detection |
| Detection Method | Dilute primary antibody to desired concentration; incubate with membrane 1-2 hr at RT or overnight at 4 °C |
| Components / Formulation | Synthetic PVP-based polymer, non-ionic surfactant blend, Tris-buffered saline (pH 7.4), EDTA (0.5 mM), azide-free preservative |
| Storage Conditions | 2-8 °C; do not freeze; protect from light |
| Shelf Life | 24 months; 12 months after opening |
| Package Specifications | 100 mL, 250 mL, 500 mL HDPE bottles |
| Product Form | Clear pale yellow liquid, ready-to-use |
| Key Features | Protein-free — no antibody cross-reactivity with blocking proteins; superior for phospho-specific antibodies; chemically defined; low background with chemiluminescent and fluorescent detection; azide-free for HRP |
| Purity | No detectable protein by BCA (<5 µg/mL); protease and phosphatase tested free |
| Concentration | Ready-to-use; no dilution required |
| Activity / Unit Definition | Dilution linearity R² ≥0.99 across specified range |
| Molecular Weight | Not applicable — buffered protein solution |
| Source / Origin | Bovine serum albumin, casein, and synthetic buffer components |
| pH Range / Optimal pH | pH 7.4 ± 0.1 at 25 °C |
| Shipping Conditions | Ambient; protect from freezing |
| Expiration Date / Stability | 24 months at 2-8 °C; 12 months after opening |
| Regulatory / Compliance | For laboratory and research use only; RUO; animal component-free; ISO 9001 |
| Compatibility | Compatible with ELISA, CLIA, and immunoturbidimetric assay platforms |
| Recommended Buffer System | PBS-based with protein stabilizers and antimicrobial preservative |
| Application Notes / Precautions | Bring to room temperature before use; avoid repeated freeze-thaw; use aseptic technique for opened bottles |
| Batch-to-Batch Consistency | Dilution recovery 90–110% for reference analyte panel per lot |
For research use only, not for clinical use.
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