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| Product Name | Enzyme Conjugate Stabilizer and Diluent, HRP/AP Formulation |
| Catalog No. | ATRD-0020 |
| Description | Protein-based conjugate stabilizer and diluent for long-term preservation of HRP and AP enzyme conjugate activity at high dilutions. Proprietary matrix of stabilizing proteins and anti-aggregation agents maintains >90% conjugate activity after 12 months at 4 °C, dramatically outperforming PBS or simple buffer dilutions. |
| Intended Use | Dilution and long-term storage of HRP/AP-conjugated antibodies, streptavidin, and detection reagents for ELISA, Western blot, IHC, and lateral flow immunoassays. |
| Principle / Technology | Molecular crowding protein matrix preserves enzyme conformation; anti-aggregation agents prevent precipitation; chelators remove inactivating heavy metals; antimicrobial preservatives prevent degradation |
| Detection Method | Dilute conjugate to working concentration; store at 4 °C up to 12 months or -20 °C up to 24 months |
| Components / Formulation | Protease-free BSA, casein hydrolysate, glycerol (5%), EDTA (1 mM), Tris buffer (50 mM, pH 7.4), NaCl (150 mM), ProClin 300 (<0.05%), anti-aggregation polymer |
| Storage Conditions | 2-8 °C; do not freeze; protect from light |
| Shelf Life | 36 months in unopened container |
| Package Specifications | 50 mL, 250 mL, 1 L HDPE bottles |
| Product Form | Clear pale yellow liquid, ready-to-use |
| Key Features | >90% conjugate activity after 12 months at 4 °C; universal HRP/AP compatibility; anti-aggregation prevents signal loss; protease-free BSA; low biotin (<1 ng/mL) for streptavidin applications |
| Purity | BSA >98%, protease-tested; heavy metals <1 ppm; biotin <1 ng/mL |
| Concentration | Ready-to-use; no dilution required |
| Activity / Unit Definition | Dilution linearity R² ≥0.99 across specified range |
| Molecular Weight | Not applicable — buffered protein solution |
| Source / Origin | Bovine serum albumin, casein, and synthetic buffer components |
| pH Range / Optimal pH | pH 7.4 ± 0.1 at 25 °C |
| Shipping Conditions | Ambient; protect from freezing; avoid >35 °C |
| Expiration Date / Stability | 36 months at 2-8 °C unopened; diluted conjugate: 12 months at 4 °C, 24 months at -20 °C |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with ELISA, CLIA, and immunoturbidimetric assay platforms |
| Recommended Buffer System | PBS-based with protein stabilizers and antimicrobial preservative |
| Application Notes / Precautions | Bring to room temperature before use; avoid repeated freeze-thaw; use aseptic technique for opened bottles |
| Batch-to-Batch Consistency | Dilution recovery 90–110% for reference analyte panel per lot |
For research use only, not for clinical use.
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