- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Fatty Acid Uptake Fluorometric Cell-Based Assay Kit |
| Catalog No. | CMTR-HMM-0067 |
| Description | A cell-based fluorometric assay kit for measuring cellular fatty acid uptake using a fluorescently labeled long-chain fatty acid analog. The fluorescent fatty acid probe is taken up by cells through both passive diffusion and protein-facilitated transport mechanisms, with uptake quantified by fluorescence intensity measurement following a wash step to remove unincorporated probe. The kit enables comparison of fatty acid transport activity across different cell types and treatment conditions. |
| Intended Use | Measurement of cellular fatty acid uptake capacity in cultured cells for metabolic and obesity research applications. |
| Principle / Technology | Cellular uptake of fluorescent long-chain fatty acid analog quantified by intracellular fluorescence |
| Detection Method | Fluorescence (excitation 488 nm, emission 520 nm) |
| Sample Type | Adipocytes, hepatocytes, skeletal muscle cells, and other cultured mammalian cells |
| Performance Range / Specifications | Uptake linear over 5–60 min incubation; signal stable for 2 hr after wash |
| Sensitivity / LOD | Detectable signal from as few as 2000 cells per well in 96-well format |
| Specificity | Fluorescent fatty acid analog (C₁₂ or C₁₆ chain) transported by fatty acid translocase and fatty acid transport proteins |
| Reaction Conditions / Protocol | Wash cells; incubate with fluorescent fatty acid probe 30 min at 37 °C; wash 3× with ice-cold PBS; read fluorescence |
| Components / Formulation | Fluorescent fatty acid probe (BODIPY-labeled, 1 mM in DMSO), fatty-acid-free BSA, HBSS wash buffer, phloretin uptake inhibitor control (optional) |
| Storage Conditions | –20 °C for probe, protected from light; 2–8 °C for buffers |
| Shelf Life | 12 months at –20 °C |
| Package Specifications | 200 tests (96-well format) |
| Product Form | Probe: DMSO solution; buffers: liquid |
| Quality Control | Probe purity ≥95% by HPLC; cell uptake validation with 3T3-L1 adipocytes |
| Key Features | Wash step removes extracellular fluorescence, providing specific intracellular uptake measurement |
| Purity | Reagent grade; fluorescent probes ≥95% pure by HPLC |
| Concentration | As specified per kit format; probe stock solutions in DMSO as specified |
| Activity / Unit Definition | Standard curve linearity R² ≥0.99 per lot; dynamic range as specified |
| Molecular Weight | Varies by probe and buffer component |
| Source / Origin | Synthetic fluorescent probes; recombinant enzymes; purified metabolite standards |
| pH Range / Optimal pH | pH 7.2–7.4 for cell-based assays; pH varies for biochemical metabolite detection |
| Shipping Conditions | Cold packs or dry ice for probes and enzymes; protect from light |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 |
| Compatibility | Compatible with fluorescence microplate readers, luminometers, and spectrophotometers |
| Recommended Buffer System | Assay-specific buffer optimized per product protocol |
| Application Notes / Precautions | Normalize signal to cell number or protein content; avoid probe photobleaching; include appropriate antioxidant controls for ROS assays |
| Batch-to-Batch Consistency | Standard curve parameters within ±15% of reference lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
