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| Product Name | Exosome Ultrafiltration Tube (500 uL, 100 kDa) |
| Catalog No. | RESK-0630 |
| Description | The Exosome Ultrafiltration Tube (500 uL, 100 kDa) is built around a polyethersulfone (PES) ultrafiltration membrane with a molecular weight cut-off of 100 kDa, offering fast ultrafiltration speed, high concentration efficiency, and high exosome recovery. Its specially designed anti-dry-spin structure effectively prevents sample loss caused by over-spinning during centrifugation, thereby ensuring a high exosome recovery rate. The concentrated target exosomes are retained in the upper chamber of the tube and are suitable for a variety of downstream applications, including electron microscopy analysis, NTA particle size analysis, proteomics analysis, and cell function assays. |
| Intended Use | Concentration of exosomes from liquid samples and removal of free dye after exosome staining; the concentrated exosomes are suitable for downstream applications such as electron microscopy analysis, NTA particle size detection, proteomics analysis, and cell function assays. |
| Principle / Technology | Membrane ultrafiltration using a 100 kDa molecular weight cut-off PES membrane. Driven by centrifugal force, solvent and small molecules pass through the membrane into the lower chamber, while exosomes larger than the cut-off are retained and concentrated in the upper chamber. |
| Sample Type | Exosome-containing liquid samples; up to 500 uL of sample can be loaded into the upper chamber per tube. |
| Performance Range / Specifications | Membrane: PES ultrafiltration membrane Molecular weight cut-off: 100 kDa Maximum sample loading volume: 500 uL Fast ultrafiltration speed, high concentration efficiency, and high exosome recovery rate Non-sterile |
| Reaction Conditions / Protocol | A. Exosome concentration 1. Add no more than 500 uL of sample into the upper chamber of the ultrafiltration tube, tighten the cap, and place the tube into the centrifuge, balancing the rotor properly. 2. Centrifuge at 3000 xg for 5-10 min at 4 °C. 3. Repeat the centrifugation until the upper chamber volume is concentrated to the desired volume. 4. Recover the concentrate from the upper chamber; aspirate the liquid slowly to avoid bubbles and recover as completely as possible. B. Removal of free dye after exosome staining 1. Add 2 volumes of PBS to the sample and use the 100 kDa ultrafiltration tube; centrifuge at 3000 xg for 5-10 min to perform ultrafiltration buffer exchange, removing free dye from the solution, and concentrate the upper chamber sample back to the original volume. 2. Repeat the ultrafiltration exchange step twice. 3. Collect the sample from the upper chamber of the ultrafiltration tube; this is the stained exosome fraction. |
| Storage Conditions | Store at room temperature. |
| Shelf Life | 2 years. |
| Package Specifications | 5 units (5T). |
| Product Form | Centrifugal ultrafiltration tube equipped with a 100 kDa PES membrane. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with downstream applications including electron microscopy analysis, NTA particle size detection, proteomics analysis, and cell function assays. |
| Recommended Buffer System | PBS is used as the exchange buffer when removing free dye after exosome staining. |
| Application Notes / Precautions | 1. Recover the concentrate from the upper chamber as soon as possible after centrifugation. 2. The liquid in the upper chamber is the concentrated exosomes; the liquid in the lower chamber is the filtrate waste. 3. Never exceed 3000 xg during centrifugation to avoid damaging the ultrafiltration membrane or reducing recovery efficiency. 4. This product is non-sterile. |
For research use only, not for clinical use.
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