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| Product Name | Exosome Purification Column |
| Catalog No. | RESK-0339 |
| Description | Exosomes are small vesicles of approximately 30-150 nm in diameter secreted by living cells, featuring a typical lipid bilayer structure. They are present in cell culture supernatants, serum, plasma, saliva, urine, amniotic fluid, and other biological fluids. Exosomes carry important cargo including proteins, lipids, DNA, and RNA; they not only play key roles in intercellular material and information transfer, but are also promising early diagnostic biomarkers for a variety of diseases. This product is a purification column dedicated to exosome isolation and can be used in combination with an exosome rapid extraction kit to further purify crude exosome pellet preparations. |
| Intended Use | Purification of crude exosome preparations isolated from serum, plasma, urine, cell culture supernatant, and other biological fluids. For research use only. |
| Principle / Technology | Size-based filtration combined with electrostatic adsorption: the column removes residual DNA by electrostatic adsorption and removes microvesicles and some contaminating proteins by pore-size exclusion; exosomes below 200 nm pass through and are collected. The 200/220 nm filter pore size removes large-particle impurities. |
| Detection Method | Column-based centrifugal filtration; downstream exosome analysis by standard methods (e.g., particle analysis, protein or nucleic acid assays). |
| Sample Type | Crude exosome pellets/particles isolated from cell culture supernatant, serum, plasma, saliva, urine, amniotic fluid, and other biological fluids. |
| Performance Range / Specifications | Filter pore size: 200 nm (220 nm filter column) Column material: polypropylene (PP), no packed resin Sample loading volume: 200 uL (dependent on sample load; excessive sample volume may clog the column) Recovery rate: approximately 60% Centrifugation: 4 °C, 3000 x g, 10 min |
| Specificity | Retains/removes particles larger than the pore size; exosomes below 200 nm are recovered in the flow-through. |
| Reaction Conditions / Protocol | 1. Extract exosomes using an exosome rapid extraction kit. 2. Transfer the harvested crude exosome pellet preparation into the upper chamber of the exosome purification column. 3. Centrifuge at 4 °C, 3000 x g for 10 min. 4. Collect the liquid at the bottom of the tube, which contains the purified exosome particles. 5. Storage of purified exosomes: keep at 4 °C for up to 3 days; for long-term storage, aliquot and store at -80 °C, avoiding repeated freeze-thaw cycles. |
| Components / Formulation | Polypropylene (PP) purification column (unpacked, no resin). |
| Storage Conditions | Store at room temperature. |
| Shelf Life | 2 years when stored as recommended. |
| Package Specifications | 10 T 20 T 30 T |
| Product Form | Single-use centrifugal purification column (polypropylene, without packed resin). |
| Shipping Conditions | Shipped at ambient temperature. |
| Expiration Date / Stability | Stable for 2 years at room temperature. |
| Compatibility | Designed for use together with exosome rapid extraction kits for the purification of crude exosome preparations. |
| Application Notes / Precautions | For single use only; the column cannot be reused. Typical loading volume is 200 uL; excessive sample volume may clog the column. Purified exosomes can be stored at 4 °C for 3 days; aliquot and store at -80 °C for long-term storage and avoid repeated freeze-thaw cycles. |
For research use only, not for clinical use.
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