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| Product Name | Exosome Isolation Reagent (Polymer-Based Precipitation Method) |
| Catalog No. | ATR-SPS-0021 |
| Description | A polymer-based precipitation reagent for the rapid isolation of intact extracellular vesicles including exosomes from cell culture media, serum, plasma, and urine. The non-ionic polymer selectively reduces the solubility of nanoscale vesicles, allowing their recovery by low-speed centrifugation without the ultracentrifugation steps required by traditional methods. The isolated vesicles retain their native morphology and surface protein composition. |
| Intended Use | Rapid precipitation-based isolation of extracellular vesicles and exosomes from biological fluids for biomarker discovery and functional studies. |
| Principle / Technology | Polymer-mediated reduction of extracellular vesicle solubility with low-speed centrifugation recovery |
| Detection Method | Nanoparticle tracking analysis, Western blot for exosomal markers, electron microscopy |
| Sample Type | Cell culture conditioned medium, serum, plasma, urine, saliva |
| Performance Range / Specifications | Typical exosome yield: 50–200 µg protein from 10 mL cell culture medium; particle size: 50–150 nm |
| Sensitivity / LOD | Detectable exosome recovery from as little as 100 µL serum |
| Specificity | Enriches vesicles expressing CD63, CD81, CD9, and Alix exosomal markers |
| Reaction Conditions / Protocol | Centrifuge sample to remove cells and debris (2000 × g, 20 min); add reagent (1:2 for culture medium, 1:4 for serum); incubate overnight at 4 °C; centrifuge 30 min at 1500 × g; resuspend pellet in PBS |
| Components / Formulation | Exosome precipitation polymer solution, resuspension buffer (PBS with protease inhibitors) |
| Storage Conditions | 2–8 °C for precipitation reagent; –20 °C for resuspension buffer |
| Shelf Life | 12 months |
| Package Specifications | 10 mL (sufficient for 20 mL culture medium or 5 mL serum processing) |
| Product Form | Liquid polymer solution |
| Quality Control | Each lot validated by NTA particle size distribution and Western blot detection of CD63, CD81, TSG101 |
| Key Features | Low-speed centrifugation workflow is accessible to any laboratory without an ultracentrifuge |
| Purity | DNase/RNase free; sterile-filtered where applicable |
| Concentration | As specified on product label |
| Activity / Unit Definition | Sample processing efficiency ≥90% recovery per validation protocol |
| Molecular Weight | Not applicable or as specified for enzyme components |
| Source / Origin | Synthetic and recombinant components as specified |
| pH Range / Optimal pH | As stated in product specification |
| Shipping Conditions | Ambient for buffers; cold packs for enzyme components |
| Expiration Date / Stability | 12–18 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 or ISO 13485 quality system |
| Compatibility | Compatible with downstream PCR, mass spectrometry, and immunoassay workflows |
| Recommended Buffer System | Use as directed; components optimized for stated application |
| Application Notes / Precautions | Work quickly to prevent sample degradation; use appropriate protease/phosphatase inhibitors if needed |
| Batch-to-Batch Consistency | Protein precipitation efficiency and analyte recovery within ±15% of reference lot |
For research use only, not for clinical use.
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