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Exosome Isolation Reagent for Serum

Cat.No: ATR-SPS-0035 Datasheet

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Product Name Exosome Isolation Reagent for Serum
Catalog No. ATR-SPS-0035
Description A serum-specific precipitation reagent for isolating exosomes with routine low-speed centrifugation. The procedure shortens processing time and minimizes the mechanical stress associated with ultracentrifugation.
Intended Use Isolation of serum-derived exosomes for protein studies, RNA analysis, high-throughput sequencing and related downstream research.
Principle / Technology Selective precipitation of serum extracellular vesicles followed by low-speed centrifugal collection.
Detection Method Downstream nanoparticle, protein, RNA and sequencing analyses.
Sample Type Serum.
Performance Range / Specifications Minimum recommended serum input: 0.5 mL. Isolated pellets may be resuspended in 1× PBS or directly treated with a downstream lysis reagent.
Reaction Conditions / Protocol Combine serum with the isolation reagent, incubate as specified and collect the exosome-rich pellet by low-speed centrifugation.
Components / Formulation Serum exosome isolation precipitation reagent.
Storage Conditions Store at 2-8°C.
Package Specifications 10 mL.
Product Form Liquid precipitation reagent.
Key Features No ultracentrifuge required; low starting-volume requirement; reduced processing time; stable vesicle recovery; morphology-preserving low-speed collection.
Compatibility Protein analysis, RNA extraction, miRNA analysis and high-throughput sequencing workflows.
Application Notes / Precautions Use at least 0.5 mL serum when sufficient downstream yield is required. Resuspend difficult pellets gently; low-speed homogenization may be used only when intact vesicle morphology is not essential.

For research use only, not for clinical use.

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