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| Product Name | Exosome Isolation Reagent for Cell Culture Supernatant |
| Catalog No. | ATR-SPS-0034 |
| Description | A one-step precipitation reagent for isolating exosomes from cell-culture supernatants using routine low-speed centrifugation. The workflow reduces mechanical stress and supports recovery of vesicles with preserved morphology. |
| Intended Use | Isolation of exosomes from cultured-cell supernatants for RNA analysis, high-throughput sequencing and cell co-culture studies. |
| Principle / Technology | Selective precipitation of extracellular vesicles followed by low-speed centrifugation. |
| Detection Method | Downstream nanoparticle, protein, RNA and sequencing analyses. |
| Sample Type | Cell-culture supernatant from a variety of cultured cell types. |
| Performance Range / Specifications | Suitable for low-volume inputs beginning at approximately 1 mL of culture supernatant. Recovered pellets may be resuspended in 100-200 µL of 1× PBS or processed directly with a downstream lysis reagent. |
| Reaction Conditions / Protocol | Add the isolation reagent to clarified culture supernatant, incubate as directed and collect the precipitated vesicles by low-speed centrifugation. |
| Components / Formulation | Exosome isolation precipitation reagent. |
| Storage Conditions | Store at 4°C. Do not freeze. |
| Package Specifications | 50 mL. |
| Product Form | Liquid precipitation reagent. |
| Key Features | No ultracentrifuge required; one-step precipitation; low-speed centrifugation; low sample-volume compatibility; preserved vesicle morphology. |
| Compatibility | RNA extraction, miRNA analysis, high-throughput sequencing and cell co-culture workflows. |
| Application Notes / Precautions | To minimize serum-derived vesicle contamination, use serum-free medium or exosome-depleted serum during the collection period. Collect supernatant after an appropriate conditioning interval and clarify it before isolation. |
For research use only, not for clinical use.
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