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| Product Name | Exosome Isolation Precipitation Reagent, PEG-Based |
| Catalog No. | ATR-SPS-0014 |
| Description | A polyethylene glycol-based precipitation reagent for the rapid and efficient isolation of extracellular vesicles and exosomes from cell culture media, serum, plasma, and urine without ultracentrifugation. |
| Intended Use | Isolation of exosomes and extracellular vesicles for RNA and protein biomarker discovery, functional studies, and diagnostic applications using standard laboratory centrifuges. |
| Principle / Technology | PEG polymers exclude water from the hydration shell of extracellular vesicles, reducing their solubility and causing precipitation at low centrifugation speeds; proprietary formulation maximizes exosome yield while minimizing co-precipitation of protein aggregates. |
| Detection Method | Nanoparticle tracking analysis (NTA) for particle size and concentration, Western blot for exosome markers CD63, CD81, CD9 |
| Sample Type | Cell culture conditioned medium, human serum, plasma, urine, cerebrospinal fluid |
| Performance Range / Specifications | Particle yield 10^9–10^11 particles per mL; size range 30–200 nm; exosome marker enrichment >10-fold |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Clarify sample by centrifugation at 2000 × g for 30 min; add precipitation reagent; mix and incubate overnight at 4°C; centrifuge at 1500 × g for 30 min; resuspend pellet in PBS. |
| Components / Formulation | Polyethylene glycol 8000, proprietary polymer mixture, in PBS |
| Storage Conditions | 2–8°C |
| Shelf Life | 12 months |
| Package Specifications | 10 mL (for 50 mL sample), 50 mL |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Particle yield by NTA, exosome marker Western blot, sterility, lot-to-lot consistency |
| Key Features | No ultracentrifugation needed; compatible with standard centrifuges; validated exosome markers; high yield; works with multiple biofluids |
For research use only, not for clinical use.
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