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| Product Name | Exosome Isolation and Purification Kit (from Tissue) |
| Catalog No. | RESK-0629 |
| Description | Exosomes are small vesicles of approximately 30-150 nm in diameter with a bilayer lipid membrane structure, secreted by living cells. They are widely distributed in various body fluids, including peripheral blood, ascites, urine, saliva and cerebrospinal fluid, and contain diverse bioactive components such as proteins, lipids and nucleic acids. Research into the biological functions of exosomes requires the isolation of intact exosomal particles. This exosome isolation and purification kit features optimized components and is suitable for exosome extraction from a variety of tissues, including brain, heart, liver, lung, muscle, spleen, lymph node, thymus, embryo and tumor tissues. It rapidly and efficiently yields high-purity exosomal particles for electron microscopy analysis, NTA particle size analysis, nucleic acid analysis, protein analysis, cell-based experiments and animal experiments. |
| Intended Use | Isolation and purification of intact exosomal particles from various tissues (including brain, heart, liver, lung, muscle, spleen, lymph node, thymus, embryo and tumor tissues) for electron microscopy analysis, NTA particle size analysis, nucleic acid analysis, protein analysis, cell-based experiments and animal experiments. |
| Principle / Technology | Optimized reagent components for the extraction of exosomes from tissue samples, followed by purification with nuclease-free, sterile exosome purification filters to rapidly obtain high-purity exosomal particles. |
| Sample Type | Tissue samples, including brain, heart, liver, lung, muscle, spleen, lymph node, thymus, embryo and tumor tissues |
| Performance Range / Specifications | In general, 0.1 g of tissue sample (taking myocardial tissue as an example) yields 100-200 μg of exosomes (5E+9-1E+10 total particles). Because exosome content differs among tissues, the yield will vary accordingly. |
| Components / Formulation | Solution A2: 5 mL (2 T) / 48 mL (20 T), store at -20 °C Solution B2: 1.2 mL (2 T) / 12 mL (20 T), room temperature Exosome purification filters: 2 (2 T) / 20 (20 T), room temperature; nuclease-free and sterile |
| Storage Conditions | Store Solution A2 at -20 °C; store other components at room temperature. |
| Shelf Life | 2 years. |
| Package Specifications | 2 T, 20 T |
| Regulatory / Compliance | For research use only. |
| Compatibility | The isolated exosomes are suitable for electron microscopy analysis, NTA particle size analysis, nucleic acid analysis, protein analysis, cell-based experiments and animal experiments. |
| Application Notes / Precautions | All components are nuclease-free and sterile. In general, 0.1 g of tissue sample (taking myocardial tissue as an example) yields 100-200 μg of exosomes (5E+9-1E+10 total particles); because exosome content differs among tissues, the yield will vary accordingly. |
For research use only, not for clinical use.
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